EFFECTS OF NOREPINEPHRINE ON THE MORPHOLOGY AND SOME ENZYME ACTIVITIES OF PRIMARY MONOLAYER CULTURES FROM RAT BRAIN

EFFECTS OF NOREPINEPHRINE ON THE MORPHOLOGY AND SOME ENZYME ACTIVITIES OF PRIMARY MONOLAYER... Abstract— Addition of norepinephrine or isoproterenol to primary cultures started from the brains of 1‐3 day old rats caused up to 200‐fold increases in cAMP levels, which reached a maximum by 5‐10 min and then declined. This effect was studied in detail for norepinephrine. The rise in cAMP levels was followed by morphological changes, in which up to 65% of the cells exhibited an astrocyte‐like morphology, and 2‐3 fold increases in carbonic anhydrase and (Na+‐K+) ATPase activities. However, morphological transformation also occurred after much smaller increases in total cAMP levels. These effects on cell morphology and enzyme activities reached a maximum 1‐2 h after addition of norepinephrine and then declined. Carbonic anhydrase activity was found both in the particulate and post 100,000 g supernatant fractions from homogenates of these cultured cells, and in the latter case the activity was activated 3‐fold by addition of cAMP. The significance of these obscrvations on the cellular localization of, and functional role for similar increases in cAMP in brain tissue is discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

EFFECTS OF NOREPINEPHRINE ON THE MORPHOLOGY AND SOME ENZYME ACTIVITIES OF PRIMARY MONOLAYER CULTURES FROM RAT BRAIN

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Publisher
Wiley
Copyright
Copyright © 1978 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1978.tb06575.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— Addition of norepinephrine or isoproterenol to primary cultures started from the brains of 1‐3 day old rats caused up to 200‐fold increases in cAMP levels, which reached a maximum by 5‐10 min and then declined. This effect was studied in detail for norepinephrine. The rise in cAMP levels was followed by morphological changes, in which up to 65% of the cells exhibited an astrocyte‐like morphology, and 2‐3 fold increases in carbonic anhydrase and (Na+‐K+) ATPase activities. However, morphological transformation also occurred after much smaller increases in total cAMP levels. These effects on cell morphology and enzyme activities reached a maximum 1‐2 h after addition of norepinephrine and then declined. Carbonic anhydrase activity was found both in the particulate and post 100,000 g supernatant fractions from homogenates of these cultured cells, and in the latter case the activity was activated 3‐fold by addition of cAMP. The significance of these obscrvations on the cellular localization of, and functional role for similar increases in cAMP in brain tissue is discussed.

Journal

Journal of NeurochemistryWiley

Published: Dec 1, 1978

References

  • Brain carbonic anhydrase: activity in isolated myelin and the effect of hexachlorophene
    Cammer, Cammer; Fredman, Fredman; Rose, Rose; Norton, Norton
  • The effect of catecholamines on Na‐K transport and membrane potential in rat soleus muscle
    Clalses, Clalses; Flatman, Flatman
  • Variability of the effects of serum‐free medium, dibutyryl‐cyclic AMP or theophylline on the morphology of cultured new‐born rat astroblasts
    Moonen, Moonen; Cam, Cam; Sensenbrenner, Sensenbrenner; Manuel, Manuel
  • Effect of serum withdrawal on Na + ‐K + ATPase activity in astrocytes cultured from dissociated brain hemispheres
    Schousboe, Schousboe; Fosmark, Fosmark; Formby, Formby
  • Cyclic AMP and cell morphology in cultured fibroblasts. Effects on cell shape, microfilament and microtubule distribution, and orientation to substratum
    Willingham, Willingham; Pastan, Pastan
  • The subcellular distribution of carbonic anhydrase in homogenates of perfused rat brain
    Yandrasitz, Yandrasitz; Ernst, Ernst; Salganicoff, Salganicoff

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