Effect of olive oil phenolic compounds on osteoblast
Francisco Javier Manzano-Moreno
Elvira De Luna-Bertos
Biomedical Group (BIO277),
Department of Nursing, Faculty of Health
Sciences, University of Granada,
ibs.Granada, Granada, Spain
Biomedical Group (BIO277),
Department of Stomatology, School of
Dentistry, University of Granada,
AGR-255 Group, Department of
Nutrition and Food Sciences, Faculty of
Pharmacy, University of Granada,
Institute of Neuroscience Federico
oriz, University of Granada, Granada,
Javier Ramos-Torrecillas, Department of
Nursing, Faculty of Health Sciences,
University of Granada, Granada, Spain.
BIO277 (Junta de Andaluc
of Nursing (University of Granada);
Spanish Ministry of Education, Grant/
Award Number: FPU15-05635
Background: Osteoporosis is a skeletal disorder characterized by compromised
bone strength that predisposes individuals to an increased risk of fracture. Previ-
ous in vivo and in vitro studies have reported that phenolic compounds present in
extra virgin olive oil have a beneficial effect on osteoblasts in terms of increase
cell proliferation. The aim of this study was to determine whether phenolic com-
pounds present in olive oil could modify the expression of cell differentiation
markers on osteoblasts.
Study design: An in vitro experimental design was performed using MG-63
osteoblasts cell line.
Methods: MG63 cells were exposed to different doses of luteolin, apigenin, or p-
coumaric, caffeic or ferulic acid. Alkaline phosphatase (ALP) was evaluated by
spectrophotometry and antigen expression (cluster of differentiation [CD] 54,
CD80, CD86 and HLA-DR) by flow cytometry.
Results: At 24 hour, treated groups showed an increased ALP and modulated
antigen profile, with respect to the nontreated group.
Conclusion: These results demonstrate that the phenolic compounds studied
induce cell maturation in vitro, increasing ALP synthesis and reducing the expres-
sion of antigens involved in immune functions of the osteoblast which would
improve bone density.
alkaline phosphatase activity, olea europaea, osteoblastic cells, phenolic compounds, phenotype profile
Bone metabolic diseases develop when there is an imbal-
ance between the formation and resorption of bone that
depends on the interaction between osteoblasts and osteo-
clasts. Osteoporosis is the most common bone metabolic
disease in adults, especially among the elderly, which is
characterized by compromised bone strength that
predisposes individuals to an increased risk of fracture.
Most current pharmacological approaches focus on
inhibiting bone resorption in patients with osteoporosis
or at risk of developing the disease.
researchers have also provided evidence on dietary com-
ponents that can optimize bone mass and stimulate bone
Thus, Habauzit & Horcajada
phytonutrients in our diet, especially polyphenols, can act
on both osteoblasts and osteoclasts to modulate bone
Received: 30 March 2017
Accepted: 29 January 2018
Eur J Clin Invest. 2018;48:e12904.
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