Effect of Nerve Growth Factor on Glucose Utilization and Nucleotide Content of Pheochromocytoma Cells (Clone PC12)

Effect of Nerve Growth Factor on Glucose Utilization and Nucleotide Content of Pheochromocytoma... Abstract: The effect of nerve growth factor (NGF) on the utilization and fate of uniformly labeled 14C glucose and on the content of several pyridine and purine nucleotides has been tested in the clonal cell line PC12. After incubation for 72 h with NGF, PC12 cells exhibit a 2.7‐fold increase in glucose utilization and a 4.7‐fold increase in CO2 release. During the same incubation period, all the nucleotides tested (NAD+, AMP, GMP, UDP‐glucose, UDP‐galactose, UDP, ADP, GDP, UTP, CTP, ATP, and GTP) underwent significant increments, varying from a minimum of 27% for ADP to a maximum of 90–120% for AMP, GMP, UDP‐glucose, and UDP‐galactose. These findings are discussed in connection with the trophic and differentiative effects of NGF in PC12 cells, which, in the presence of this factor, shifted from a neoplastic to a neu‐ronal‐like cell population. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Effect of Nerve Growth Factor on Glucose Utilization and Nucleotide Content of Pheochromocytoma Cells (Clone PC12)

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1986.tb04512.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: The effect of nerve growth factor (NGF) on the utilization and fate of uniformly labeled 14C glucose and on the content of several pyridine and purine nucleotides has been tested in the clonal cell line PC12. After incubation for 72 h with NGF, PC12 cells exhibit a 2.7‐fold increase in glucose utilization and a 4.7‐fold increase in CO2 release. During the same incubation period, all the nucleotides tested (NAD+, AMP, GMP, UDP‐glucose, UDP‐galactose, UDP, ADP, GDP, UTP, CTP, ATP, and GTP) underwent significant increments, varying from a minimum of 27% for ADP to a maximum of 90–120% for AMP, GMP, UDP‐glucose, and UDP‐galactose. These findings are discussed in connection with the trophic and differentiative effects of NGF in PC12 cells, which, in the presence of this factor, shifted from a neoplastic to a neu‐ronal‐like cell population.

Journal

Journal of NeurochemistryWiley

Published: Aug 1, 1986

References

  • The nerve growth factor: biochemistry, synthesis and mechanism of action
    Greene, Greene; Shooter, Shooter
  • ( 14 C)Glucose metabolism in sympathetic ganglia of chicken embryos and in primary cultures of neurons and of other cells from these ganglia
    Larrabee, Larrabee
  • Alterations of lipid metabolism in response to nerve growth factor
    Traynor, Traynor; Shubert, Shubert; Allen, Allen

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