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- Publisher
- Wiley
- Copyright
- Copyright © 2002 Wiley Periodicals, Inc., A Wiley Company
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- DOI
- 10.1002/bit.10189
- Publisher site
- See Article on Publisher Site
Abstract
A comparison of the effects of laminar versus turbulent flow regime on the characteristics of a single‐species biofilm is presented. The study was carried out by growing Pseudomonas fluorescens biofilms in a flow cell and studying the different layers of the biological matrix with a confocal laser‐scanning microscope. The following conclusions were obtained: i) a higher concentration of cells was found in the upper layers of the microbial films than in their inner layers, regardless of the flow regime; ii) the fraction of cells in the overall biofilm mass decreased with time as the film grew; and iii) under laminar flow the total number of cells was higher than in biofilms formed under turbulent flow, but the latter had a higher number of cells per unit volume. Such conclusions, together with the fact that the biofilms were more dense and stable when formed in contact with turbulent flows, favor the design of more compact and efficient biofilm reactors operating in turbulent conditions. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 164–171, 2002; DOI 10.1002/bit.10189
Journal
Biotechnology and Bioengineering – Wiley
Published: Apr 20, 2002
Keywords: biofilm architecture; hydrodynamic conditions; laminar and turbulent flows; CLSM image analysis; biovolumes; Pseudomonas fluorescens