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N. Vardaxis, M. Boon, J. Ruijgrok (1996)
Calcification of cross-linked collagen-elastin membrane implants in vivo and their proposed use in bone regeneration.Biomaterials, 17 15
N. Niederhoffer, I. Lartaud‐Idjouadiene, P. Giummelly, C. Duvivier, R. Peslin, J. Atkinson (1997)
Calcification of medial elastic fibers and aortic elasticity.Hypertension, 29 4
N. Vyavahare, M. Ogle, F. Schoen, R. Levy (1999)
Elastin calcification and its prevention with aluminum chloride pretreatment.The American journal of pathology, 155 3
C. Schmidt, J. Baier (2000)
Acellular vascular tissues: natural biomaterials for tissue repair and tissue engineering.Biomaterials, 21 22
N. Vyavahare, D. Hirsch, E. Lerner, J. Baskin, F. Schoen, R. Bianco, H. Kruth, R. Zand, R. Levy (1997)
Prevention of bioprosthetic heart valve calcification by ethanol preincubation. Efficacy and mechanisms.Circulation, 95 2
L. Maxwell, J. Gavin, B. Barratt‐Boyes (1989)
Differences Between Heart Valve Allografts and Xenografts in the Incidence and Initiation of Dystrophic CalcificationPathology, 21
Meaney (1995)
Mechanical properties of implantable biomaterialsClin Podiatric Med Surg, 12
W. Paule, S. Bernick, B. Strates, M. Nimni (1992)
Calcification of implanted vascular tissues associated with elastin in an experimental animal model.Journal of biomedical materials research, 26 9
T. Franz (1975)
Percutaneous absorption on the relevance of in vitro data.The Journal of investigative dermatology, 64 3
Schiffmann (1969)
Amino acids at the nucleating site in mineralizing tissueCalc Tiss Res, 8
C. Lee, N. Vyavahare, R. Zand, H. Kruth, F. Schoen, R. Bianco, R. Levy (1998)
Inhibition of aortic wall calcification in bioprosthetic heart valves by ethanol pretreatment: biochemical and biophysical mechanisms.Journal of biomedical materials research, 42 1
D. Urry, J. Krivacic, J. Haider (1971)
Calcium ion effects a notable change in elastin conformation by interacting at neutral sites.Biochemical and biophysical research communications, 43 1
Ruud Bank, Michael Soudry, A. Maroudas, Joseph Mizrahi, J. Tekoppele (2000)
The increased swelling and instantaneous deformation of osteoarthritic cartilage is highly correlated with collagen degradation.Arthritis and rheumatism, 43 10
S. Truter, J. Rosenbaum-Fiedler, A. Sapadin, M. Lebwohl (1996)
Calcification of elastic fibers in pseudoxanthoma elasticum.The Mount Sinai journal of medicine, New York, 63 3-4
F. Schroeder, W. Morrison, C. Gorka, W. Wood (1988)
Transbilayer effects of ethanol on fluidity of brain membrane leaflets.Biochimica et biophysica acta, 946 1
R. Elliott, L. McGrath (1994)
Calcification of the human thoracic aorta during agingCalcified Tissue International, 54
M. Soylu, A. Demir, K. Arda, Yücel Uzun, S. Göksel (2001)
Association Between Mitral Annular Calcification and Carotid AtheromaAngiology, 52
W. Klemm (1998)
Biological water and its role in the effects of alcohol.Alcohol, 15 3
Fell (1971)
Determination of tablet strength by diametrical compression testJ Pharm Sci., 69
Y. Bobryshev, R. Lord, B. Warren (1995)
Calcified deposit formation in intimal thickenings of the human aorta.Atherosclerosis, 118 1
F. Schoen, R. Levy, S. Hilbert, R. Bianco (1992)
Antimineralization treatments for bioprosthetic heart valves. Assessment of efficacy and safety.The Journal of thoracic and cardiovascular surgery, 104 5
The role of elastin in the aortic wall calcification and involved mechanisms were investigated. The major hypothesis of this work is that elastin is one of the major components to regulate calcification of bioprosthetic heart valve (BHV). The relationship between the elastin content and the calcification rate of the aortic wall was established using collagen‐elastin matrices (CEM) made of varying ratios of collagen and elastin (90 and 10, 50 and 50, and 20 and 80). Biophysical characterization of CEM was performed by water content measurement and the tensile strength test. The conformational changes of the calcifiable matrix were evaluated as a function of elastin content using Fourier transform infrared (FTIR) spectroscopy. The calcium contents in CEM implanted in the rat subcutaneous model for 7 days were measured using atomic absorption (AA) spectroscopy. As the concentration of elastin in CEM increased from 10 to 80%, the total amount of calcium accumulated in CEM also increased. The calcium level in CEM containing the collagen and an elastin ratio of 20:80 was 20.16 ± 0.70 μg/mg compared with 1.96 ± 0.04 μg/mg in CEM containing the collagen and an elastin ratio of 90:10. The calcification rate of CEM pretreated with ethanol increased, as the elastin concentration in the CEM. However, the calcification rate of CEM pretreated with ethanol is significantly lower than that of the untreated control. The permeation rate of ethanol through CEM with the collagen and an elastin ratio of 20:80 (0.37 ± 0.13 mmol/cm2/h) is significantly smaller than that through CEM with 90:10 (0.94 ± 0.27 mmol/cm2/h). These results indicate that elastin has a significant role in tissue calcification and that elastin's resistance to ethanol penetration partially contributes less effectiveness of ethanol on aortic wall calcification. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 60: 368–374, 2002; DOI 10.1002/jbm.10077
Journal of Biomedical Materials Research Part A – Wiley
Published: May 5, 2002
Keywords: ; ; ; ;
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