Dopamine Receptor Stimulation Does Not Affect Phosphoinositide Hydrolysis in Slices of Rat Striatum

Dopamine Receptor Stimulation Does Not Affect Phosphoinositide Hydrolysis in Slices of Rat Striatum Abstract: The effect of dopamine receptor stimulation on the accumulation of labelled inositol phosphates in rat striatal slices under basal and stimulated conditions was examined following preincubation with (3H)inositol. Incubation of striatal slices with the selective D‐1 agonist SKF 38393 or the selective D‐2 agonist LY 171555 for 5 or 30 min did not affect the basal accumulation of labelled inositol mono‐, bis‐, tris‐, and tetrakisphosphate. Resolution by HPLC of inositol trisphosphate into inositol‐1,3,4‐tris‐phosphate and inositol‐1,4,5‐trisphosphate isomers revealed that under basal conditions dopamine did not influence the accumulation of inositol‐1,4,5‐trisphosphate. Depolarisation evoked by KC1, or addition of the muscarinic receptor agonist carbachol, produced a marked increase in the accumulation of labelled inositol phosphates in both the presence and absence of lithium. Addition of dopamine did not reduce the ability of KC1 or carbachol to increase inositol phospholipid hydrolysis. In the presence of lithium, dopamine (100 μM) enhanced KCl‐stimuIated inositol phospholipid hydrolysis, but this effect appears to be mediated by α1 adrenoceptors because it was blocked by prazosin. SKF 38393 (10 μM) or LY 171555 (10 μM) also did not affect carbachol‐stimulated inositol phospholipid hydrolysis. These data, in contrast to recent reports, suggest that striatal dopamine receptors do not appear to be linked to inositol phospholipid hydrolysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Dopamine Receptor Stimulation Does Not Affect Phosphoinositide Hydrolysis in Slices of Rat Striatum

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Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1988.tb01828.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: The effect of dopamine receptor stimulation on the accumulation of labelled inositol phosphates in rat striatal slices under basal and stimulated conditions was examined following preincubation with (3H)inositol. Incubation of striatal slices with the selective D‐1 agonist SKF 38393 or the selective D‐2 agonist LY 171555 for 5 or 30 min did not affect the basal accumulation of labelled inositol mono‐, bis‐, tris‐, and tetrakisphosphate. Resolution by HPLC of inositol trisphosphate into inositol‐1,3,4‐tris‐phosphate and inositol‐1,4,5‐trisphosphate isomers revealed that under basal conditions dopamine did not influence the accumulation of inositol‐1,4,5‐trisphosphate. Depolarisation evoked by KC1, or addition of the muscarinic receptor agonist carbachol, produced a marked increase in the accumulation of labelled inositol phosphates in both the presence and absence of lithium. Addition of dopamine did not reduce the ability of KC1 or carbachol to increase inositol phospholipid hydrolysis. In the presence of lithium, dopamine (100 μM) enhanced KCl‐stimuIated inositol phospholipid hydrolysis, but this effect appears to be mediated by α1 adrenoceptors because it was blocked by prazosin. SKF 38393 (10 μM) or LY 171555 (10 μM) also did not affect carbachol‐stimulated inositol phospholipid hydrolysis. These data, in contrast to recent reports, suggest that striatal dopamine receptors do not appear to be linked to inositol phospholipid hydrolysis.

Journal

Journal of NeurochemistryWiley

Published: Sep 1, 1988

References

  • Differential effects of lithium on muscarinic receptor stimulation of inositol phosphates in rat cerebral cortex slices
    Batty, Batty; Nahorski, Nahorski
  • Inositol phospholipid hydrolysis in rat cerebral cortical slices. I. Receptor characterisation
    Brown, Brown; Kendall, Kendall; Nahorski, Nahorski
  • Activation of dopamine receptors does not affect phosphoinositide turnover in NCB‐20 cells
    Cubitt, Cubitt; Brown, Brown; Dobson, Dobson
  • Muscarinic receptors and hydrolysis of inositol phospholipids in rat cerebral cortex and parotid gland
    Jacobson, Jacobson; Wusteman, Wusteman; Downes, Downes
  • Multiple receptors for dopamine
    Kebabian, Kebabian; Calne, Calne
  • Inositol phospholipid hydrolysis in rat cerebral cortical slices. II. Calcium requirement
    Kendall, Kendall; Nahorski, Nahorski
  • Pertussis toxin attenuates D‐2 inhibition and enhances D‐1 stimulation of adenylate cyclase by dopamine in rat striatum
    Olianas, Olianas; Onali, Onali

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