DNA polymorphism in the major histocompatibility complex of man and various farm animals*

DNA polymorphism in the major histocompatibility complex of man and various farm animals* Summary. In the past few years it has been possible by combining enzymatic cleavage of genomic DNA and the Southern blot hybridization technique to explore the endonuclease recognition site polymorphism of the MHC. HLA class I and DR and DQ alpha and beta class II specific probes as well as human C4 and Bf class III probes were used. All these probes were shown to cross‐hybridize with DNA from pigs, cattle, sheep and horses. Hybridization of human genomic DNA with a class I probe showed 15–25 bands per genome depending on the enzyme used. Distinct endonucleases generated clusters of restriction fragments (RF) in HLA‐informative families which correlated with HLA specificites. While numerous clusters were found associated with HLA‐A alleles almost no cluster was related to HLA B or C specificities. Similarly, class II probes provided a large number of clusters. The existence of these clusters suggested that some polymorphic restriction sites are found in strong linkage disequilibrium and that the underlying mechanism might be gene conversion with heteroduplex correction. Since the degree of polymorphism detected by RF appears to be greater than the polymorphism defined by more traditional methods stronger associations between RF and pathological conditions are to be expected. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Genetics Wiley

DNA polymorphism in the major histocompatibility complex of man and various farm animals*

Animal Genetics, Volume 17 (4) – Aug 1, 1986

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Publisher
Wiley
Copyright
Copyright © 1986 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0268-9146
eISSN
1365-2052
DOI
10.1111/j.1365-2052.1986.tb00732.x
Publisher site
See Article on Publisher Site

Abstract

Summary. In the past few years it has been possible by combining enzymatic cleavage of genomic DNA and the Southern blot hybridization technique to explore the endonuclease recognition site polymorphism of the MHC. HLA class I and DR and DQ alpha and beta class II specific probes as well as human C4 and Bf class III probes were used. All these probes were shown to cross‐hybridize with DNA from pigs, cattle, sheep and horses. Hybridization of human genomic DNA with a class I probe showed 15–25 bands per genome depending on the enzyme used. Distinct endonucleases generated clusters of restriction fragments (RF) in HLA‐informative families which correlated with HLA specificites. While numerous clusters were found associated with HLA‐A alleles almost no cluster was related to HLA B or C specificities. Similarly, class II probes provided a large number of clusters. The existence of these clusters suggested that some polymorphic restriction sites are found in strong linkage disequilibrium and that the underlying mechanism might be gene conversion with heteroduplex correction. Since the degree of polymorphism detected by RF appears to be greater than the polymorphism defined by more traditional methods stronger associations between RF and pathological conditions are to be expected.

Journal

Animal GeneticsWiley

Published: Aug 1, 1986

Keywords: ; ; ; ; ; ; ; ; ; ; ; ; ;

References

  • Molecular genetic analyses of the major histocompatibility complex in pig families and recombinants
    Chardon, P.; Renard, Ch.; Kirszenbaum, M.; Geffrotin, CI.; Cohen, D.; Vaiman, M.

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