DNA decontamination methods for internal quality management in clinical PCR laboratories

DNA decontamination methods for internal quality management in clinical PCR laboratories INTRODUCTIONPolymerase chain reaction (PCR) has been developed to identify multiple pathogens in clinical specimens, which has revolutionized diagnostic approaches in a variety of fields. However, the high sensitivity of PCR has a corresponding limitation: the possibility of amplifying non‐specific products, creating so‐called DNA contamination, leading to false‐positive results. When a pathogen's nucleic acids are falsely recorded as present in a clinical molecular biology laboratory, therapeutic decisions could be affected. DNA contamination can originate from several different sources, and it is problematic when high copy numbers of DNA exist in the air or on the surfaces of objects in a laboratory.Different contamination sources require different methods of treatment. DNA contamination of laboratory surfaces and instruments from various sources can occur at any stage of an operation and can be avoided by ensuring that rooms stay clean and by using decontamination methods, such as UV‐irradiation and application of disinfectants containing alcohol and chlorine. Amplicons from the most common sources of contamination are produced at very high copy numbers during PCR and can seriously impact diagnostic analysis because they can be homologous to target molecules and amplified at high efficiency, resulting in carry‐over contamination. Contamination of PCR reagents and DNA extraction kits http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

DNA decontamination methods for internal quality management in clinical PCR laboratories

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22290
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONPolymerase chain reaction (PCR) has been developed to identify multiple pathogens in clinical specimens, which has revolutionized diagnostic approaches in a variety of fields. However, the high sensitivity of PCR has a corresponding limitation: the possibility of amplifying non‐specific products, creating so‐called DNA contamination, leading to false‐positive results. When a pathogen's nucleic acids are falsely recorded as present in a clinical molecular biology laboratory, therapeutic decisions could be affected. DNA contamination can originate from several different sources, and it is problematic when high copy numbers of DNA exist in the air or on the surfaces of objects in a laboratory.Different contamination sources require different methods of treatment. DNA contamination of laboratory surfaces and instruments from various sources can occur at any stage of an operation and can be avoided by ensuring that rooms stay clean and by using decontamination methods, such as UV‐irradiation and application of disinfectants containing alcohol and chlorine. Amplicons from the most common sources of contamination are produced at very high copy numbers during PCR and can seriously impact diagnostic analysis because they can be homologous to target molecules and amplified at high efficiency, resulting in carry‐over contamination. Contamination of PCR reagents and DNA extraction kits

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

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