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Z. Darżynkiewicz, F. Tragnos, T. Sharpless, C. Friend, M. Melamed (1976)
Nuclear chromatin changes during erythroid differentiation of friend virus induced leukemic cells.Experimental cell research, 99 2
S. Latt, Y. George, J. Gray (1977)
Flow cytometric analysis of bromodeoxyuridine-substituted cells stained with 33258 Hoechst.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25
D. Swartzendruber (1977)
A bromodeoxyuridine (BUdR)-mithramycin technique for detecting cycling and non-cycling cells by flow microfluorometry.Experimental cell research, 109 2
S. Latt, G. Stetten (1976)
Spectral studies on 33258 Hoechst and related bisbenzimidazole dyes useful for fluorescent detection of deoxyribonucleic acid synthesis.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 24
Z. Darżynkiewicz, F. Traganos, M. Melamed (1980)
New cell cycle compartments identified by multiparameter flow cytometry.Cytometry, 1 2
J. Kapuściński, Zbigniew Darzynkiewicz, Myron Melamed (2005)
Luminescence of the solid complexes of acridine orange with RNA.Cytometry, 2 4
Zbigniew Darzynkiewicz, F. Traganos, T. Sharpless, Myron Melamed (1975)
Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometry.Experimental cell research, 90 2
D. Bradley, M. Wolf (1959)
AGGREGATION OF DYES BOUND TO POLYANIONS.Proceedings of the National Academy of Sciences of the United States of America, 45 7
L. Lerman (1963)
The structure of the DNA-acridine complex.Proceedings of the National Academy of Sciences of the United States of America, 49
H. Gratzner, R. Leif (1981)
An immunofluorescence method for monitoring DNA synthesis by flow cytometry.Cytometry, 1 6
Z. Darżynkiewicz, M. Andreeff, F. Traganos, T. Sharpless, M. Melamed (1978)
Discrimination of cycling and non-cycling lymphocytes by BUdR-suppressed acridine orange fluorescence in a flow cytometric system.Experimental cell research, 115 1
S. Latt (1974)
DETECTION OF DNA SYNTHESIS IN INTERPHASE NUCLEI BY FLUORESCENCE MICROSCOPYThe Journal of Cell Biology, 62
R. Bohmer (1979)
FLOW CYTOMETRIC CELL CYCLE ANALYSIS USING THE QUENCHING OF 33258 HOECHST FLUORESCENCE BY BROMODEOXYURIDINE INCORPORATIONCell Proliferation, 12
S. Latt (1977)
Fluorometric detection of deoxyribonucleic acid synthesis; possibilities for interfacing bromodeoxyuridine dye techniques with flow fluorometry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25
SA Latt (1979)
Flow Cytometry and Sorting
Z. Darżynkiewicz, F. Traganos, T. Sharpless, M. Melamed (1977)
Recognition of cells in mitosis by flow cytofluormetry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 25
Exponentially growing Friend leukemia cells are exposed to 5‐bromodeoxyuridine (BrdUrd) for the time period equivalent to one generation. After fixation and incubation with RNase, DNA in situ is partially denatured by acid and the cells are stained with acridine orange (AO). Staining with AO under these conditions reveals the extent of denatured versus double stranded DNA and enables one to distinguish mitotic from interphase cells. It is observed that all BrdUrd treated cells, regardless of cell cycle phase, have decreased both green and red fluorescence. This finding suggests that BrdUrd interacts with AO not only in the complexes of the dye with double stranded DNA, but also with the single stranded biopolymer. The BrdUrd‐attributed suppression of cell fluorescence is large enough to separate totally BrdUrd labeled from unlabeled mitotic populations. The combination of these two flow cytometric techniques, one which allows discrimination between interphase and metaphase cells and the other which allows further subdivision of metaphase cells into those which have incorporated BrdUrd and those which have not, provides an alternative to the autoradiographic procedure necessary for obtaining the fraction of labeled mitoses.
Cytometry Part A – Wiley
Published: Mar 1, 1983
Keywords: ; ; ; ; ;
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