Discovery and analysis of a novel parvovirus B19 Genotype 3 isolate in the United States

Discovery and analysis of a novel parvovirus B19 Genotype 3 isolate in the United States BACKGROUND: Parvovirus B19 (B19V) is a pathogen frequently identified in human plasma donations through the detection of nucleic acids. Three B19V genotypes have been defined based on isolates having greater than 10% divergence in overall DNA sequence. B19V Genotype 3 is a rarely occurring genotype that has been detected primarily in Ghana with sporadic reports in Brazil and France but has not been previously reported in North America. STUDY DESIGN AND METHODS: A polymerase chain reaction assay was developed with broad specificity for B19V detection. The performance of this assay was assessed by testing approximately 440,000 clinical samples representing more than 81,000 individual donors. Determinations of B19V titer, DNA sequence, and antibody concentrations were performed on samples of interest. RESULTS: This assessment identified a series of eight plasma donations spanning 28 days from a single donor in the United States infected with B19V Genotype 3 as confirmed by DNA sequence analysis. The B19V titer of this series of donations showed virus titers that peaked at greater than 1011 IU/mL. The virus titer decreased significantly over the next several donations coinciding with an increase in immunoglobulin M (IgM) levels. The immunoglobulin G levels also increased but lagged approximately 7 days behind the IgM levels. CONCLUSION: This is the first report of a B19V Genotype 3 detected from a plasma donor located in the United States. Although our data are consistent with recent reports suggesting low incidence for this genotype, they indicate its increasing relevance among blood and plasma donors. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Discovery and analysis of a novel parvovirus B19 Genotype 3 isolate in the United States

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Publisher
Wiley
Copyright
© 2009 American Association of Blood Banks
ISSN
0041-1132
eISSN
1537-2995
D.O.I.
10.1111/j.1537-2995.2009.02160.x
Publisher site
See Article on Publisher Site

Abstract

BACKGROUND: Parvovirus B19 (B19V) is a pathogen frequently identified in human plasma donations through the detection of nucleic acids. Three B19V genotypes have been defined based on isolates having greater than 10% divergence in overall DNA sequence. B19V Genotype 3 is a rarely occurring genotype that has been detected primarily in Ghana with sporadic reports in Brazil and France but has not been previously reported in North America. STUDY DESIGN AND METHODS: A polymerase chain reaction assay was developed with broad specificity for B19V detection. The performance of this assay was assessed by testing approximately 440,000 clinical samples representing more than 81,000 individual donors. Determinations of B19V titer, DNA sequence, and antibody concentrations were performed on samples of interest. RESULTS: This assessment identified a series of eight plasma donations spanning 28 days from a single donor in the United States infected with B19V Genotype 3 as confirmed by DNA sequence analysis. The B19V titer of this series of donations showed virus titers that peaked at greater than 1011 IU/mL. The virus titer decreased significantly over the next several donations coinciding with an increase in immunoglobulin M (IgM) levels. The immunoglobulin G levels also increased but lagged approximately 7 days behind the IgM levels. CONCLUSION: This is the first report of a B19V Genotype 3 detected from a plasma donor located in the United States. Although our data are consistent with recent reports suggesting low incidence for this genotype, they indicate its increasing relevance among blood and plasma donors.

Journal

TransfusionWiley

Published: Jul 1, 2009

References

  • Effects of probe binding mutations in an assay designed to detect parvovirus B19: implications for the quantitation of different virus genotypes
    Baylis, Baylis; Fryer, Fryer; Grabarczyk, Grabarczyk

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