dim‐2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora

dim‐2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora To understand better the control of DNA methylation, we cloned and characterized the dim‐2 gene of Neurospora crassa, the only eukaryotic gene currently known in which mutations appear to eliminate DNA methylation. The dim‐2 gene is responsible for methylation in both symmetrical and asymmetrical sites. We mapped dim‐2 between wc‐1 and un‐10 on linkage group (LG) VIIR and identified the gene by RFLP mapping and genetic complementation. Dim‐2 encodes a 1454 amino acid protein including a C‐terminal domain homologous to known DNA methyltransferases (MTases) and a novel N‐terminal domain. Neither a deletion that removed the first 186 amino acids of the protein nor a mutation in a putative nucleotide binding site abolished function, but a single amino acid substitution in the predicted catalytic site did. Tests for repeat‐induced point mutation (RIP) indicated that dim‐2 does not play a role in this process, i.e. duplicated sequences are mutated in dim‐2 strains, as usual, but the mutated sequences are not methylated, unlike the situation in dim‐2+ strains. We conclude that dim‐2 encodes an MTase that is responsible for all DNA methylation in vegetative tissues of Neurospora. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

dim‐2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora

The EMBO Journal, Volume 20 (15) – Aug 1, 2001

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Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
D.O.I.
10.1093/emboj/20.15.4309
Publisher site
See Article on Publisher Site

Abstract

To understand better the control of DNA methylation, we cloned and characterized the dim‐2 gene of Neurospora crassa, the only eukaryotic gene currently known in which mutations appear to eliminate DNA methylation. The dim‐2 gene is responsible for methylation in both symmetrical and asymmetrical sites. We mapped dim‐2 between wc‐1 and un‐10 on linkage group (LG) VIIR and identified the gene by RFLP mapping and genetic complementation. Dim‐2 encodes a 1454 amino acid protein including a C‐terminal domain homologous to known DNA methyltransferases (MTases) and a novel N‐terminal domain. Neither a deletion that removed the first 186 amino acids of the protein nor a mutation in a putative nucleotide binding site abolished function, but a single amino acid substitution in the predicted catalytic site did. Tests for repeat‐induced point mutation (RIP) indicated that dim‐2 does not play a role in this process, i.e. duplicated sequences are mutated in dim‐2 strains, as usual, but the mutated sequences are not methylated, unlike the situation in dim‐2+ strains. We conclude that dim‐2 encodes an MTase that is responsible for all DNA methylation in vegetative tissues of Neurospora.

Journal

The EMBO JournalWiley

Published: Aug 1, 2001

References

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