Digital image analysis agrees with visual estimates of adult bone marrow trephine biopsy cellularity

Digital image analysis agrees with visual estimates of adult bone marrow trephine biopsy cellularity INTRODUCTIONVisual bone marrow trephine biopsy examination is used routinely to evaluate the architectural distribution and composition of the marrow space. It includes assessment of cellularity of the hematopoietic and stromal compartments, and localization and extent of pathological foci whether primary hematologic or secondary. In evaluating cellularity, the standard practice is to express the results as visual estimates (VE) of percentage of surface area occupied by nonfat hematopoietic tissue. Histologic evaluation allows for a rapid, but rough semiquantitative estimate.Morphometric point counting is an accurate and direct method of bone marrow cellularity quantitation and has been correlated with image analysis methods. However, it is tedious and time‐consuming. Although point counting may be considered the gold standard, an experienced observer can usually make an estimate by VE within 5%‐10% of the value determined by the point‐counting method. Fong et al confirmed that although both point counting and VE are prone to interobserver variability, VE is a reliable form of measurement and can be completed in a fraction of the time needed for point counting. Single observer determinations of cellularity correlated highly between the two techniques, whether the specimen was a trephine biopsy, aspirate smear, or clot biopsy.Knowledge of normal variations of bone marrow http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Laboratory Hematology Wiley

Digital image analysis agrees with visual estimates of adult bone marrow trephine biopsy cellularity

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons Ltd
ISSN
1751-5521
eISSN
1751-553X
D.O.I.
10.1111/ijlh.12768
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONVisual bone marrow trephine biopsy examination is used routinely to evaluate the architectural distribution and composition of the marrow space. It includes assessment of cellularity of the hematopoietic and stromal compartments, and localization and extent of pathological foci whether primary hematologic or secondary. In evaluating cellularity, the standard practice is to express the results as visual estimates (VE) of percentage of surface area occupied by nonfat hematopoietic tissue. Histologic evaluation allows for a rapid, but rough semiquantitative estimate.Morphometric point counting is an accurate and direct method of bone marrow cellularity quantitation and has been correlated with image analysis methods. However, it is tedious and time‐consuming. Although point counting may be considered the gold standard, an experienced observer can usually make an estimate by VE within 5%‐10% of the value determined by the point‐counting method. Fong et al confirmed that although both point counting and VE are prone to interobserver variability, VE is a reliable form of measurement and can be completed in a fraction of the time needed for point counting. Single observer determinations of cellularity correlated highly between the two techniques, whether the specimen was a trephine biopsy, aspirate smear, or clot biopsy.Knowledge of normal variations of bone marrow

Journal

International Journal of Laboratory HematologyWiley

Published: Jan 1, 2018

Keywords: ; ;

References

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