Differential Regulation of Calcitonin Gene‐related Peptide (CGRP) in Regenerating Rat Facial Nucleus and Dorsal Root Ganglion

Differential Regulation of Calcitonin Gene‐related Peptide (CGRP) in Regenerating Rat Facial... The content of calcitonin gene‐related peptide (CGRP) and CGRP‐mRNA were determined in axotomized rat facial motor nucleus and sensory fifth lumbar dorsal root ganglion (L5 DRG) using radioimmunoassay and Northern blot analysis. After facial nerve transection CGRP levels in the facial nucleus showed a biphasic, five‐fold increase. A first peak occurred at postoperative day 3 and, after a transient decrease to normal levels at day 9, another increase was observed reaching a peak around the time of reinnervation (postoperative day 21). CGRP‐mRNA showed a similar, biphasic increase. The first peak in CGRP mRNA preceded the peptide peak by 2 days, the second peak was day 21. In contrast, a decrease in CGRP levels is seen in L5 DRG after sciatic nerve section, reaching minimal levels of 45% of control during the second postoperative week. CGRP‐mRNA in axotomized DRG also decreases preceding the decrease in peptide levels. No recovery to normal levels is seen for either peptide or mRNA levels in regenerating DRG up to 45 days after injury. Thus, axotomy leads to a differential regulation of both CGRP and CGRP‐mRNA in regenerating facial motor nucleus and sensory L5 DRG. This difference may be due to different regulating factors present in both the respective target tissues and the CNS regions and could reflect different functions of CGRP in regenerating motor and sensory neurons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Differential Regulation of Calcitonin Gene‐related Peptide (CGRP) in Regenerating Rat Facial Nucleus and Dorsal Root Ganglion

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Publisher
Wiley
Copyright
Copyright © 1991 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.1460-9568.1991.tb00820.x
Publisher site
See Article on Publisher Site

Abstract

The content of calcitonin gene‐related peptide (CGRP) and CGRP‐mRNA were determined in axotomized rat facial motor nucleus and sensory fifth lumbar dorsal root ganglion (L5 DRG) using radioimmunoassay and Northern blot analysis. After facial nerve transection CGRP levels in the facial nucleus showed a biphasic, five‐fold increase. A first peak occurred at postoperative day 3 and, after a transient decrease to normal levels at day 9, another increase was observed reaching a peak around the time of reinnervation (postoperative day 21). CGRP‐mRNA showed a similar, biphasic increase. The first peak in CGRP mRNA preceded the peptide peak by 2 days, the second peak was day 21. In contrast, a decrease in CGRP levels is seen in L5 DRG after sciatic nerve section, reaching minimal levels of 45% of control during the second postoperative week. CGRP‐mRNA in axotomized DRG also decreases preceding the decrease in peptide levels. No recovery to normal levels is seen for either peptide or mRNA levels in regenerating DRG up to 45 days after injury. Thus, axotomy leads to a differential regulation of both CGRP and CGRP‐mRNA in regenerating facial motor nucleus and sensory L5 DRG. This difference may be due to different regulating factors present in both the respective target tissues and the CNS regions and could reflect different functions of CGRP in regenerating motor and sensory neurons.

Journal

European Journal of NeuroscienceWiley

Published: Apr 1, 1991

References

  • Peripheral nerve section induces increased levels of calcitonin gene‐related peptide (CGRP)‐like immunoreactivity in axotomized motoneurons
    Arvidsson, Arvidsson; Johnson, Johnson; Piehl, Piehl; Cullheim, Cullheim; Hökfelt, Hökfelt; Risling, Risling; Terenius, Terenius; Ulfhake, Ulfhake
  • Displacement of synaptic terminals from regenerating motoneurons by microglial cells
    Blinzinger, Blinzinger; Kreutzberg, Kreutzberg
  • Peripheral nerve regeneration
    Fawcett, Fawcett; Keynes, Keynes
  • Calcitonin gene‐related peptide stimulates the induction of c‐fos gene expression in rat astrocyte cultures
    Haas, Haas; Reddington, Reddington; Kreutzberg, Kreutzberg
  • Primary sensory neurons of the rat showing calcitonin gene‐related peptide immunoreactivity and their relation to substance P‐, somatostatin‐, galanin‐, vasoactive intestinal polypeptide‐and cholecystokinin‐immunoreactive ganglion cells
    Ju, Ju; Hökfelt, Hökfelt; Brodin, Brodin; Fahrenkrug, Fahrenkrug; Fischer, Fischer; Frey, Frey; Elde, Elde; Brown, Brown

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