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Dielectric relaxation of DNA solutions. III. Effects of DNA concentration, protein contamination, and mixed solvents

Dielectric relaxation of DNA solutions. III. Effects of DNA concentration, protein contamination,... As a continuation of previous papers (Biopolymers (1976) 15, 879; (1978) 17, 1508), the low‐frequency dielectric relaxation of DNA solutions was studied with a four‐electrode cell and the simultaneous two‐frequency measurement. Below a critical concentration, the dielectric relaxation time agrees with the rotational relaxation time estimated from the reduced viscosity and is almost independent of DNA concentration Cp, and the dielectric increment is proportional to Cp. The critical concentration is approximately 0.02% of DNA for molecular weight Mr 2 × 106 and 0.2% for Mr 4.5 × 105 in 1 mM NaCl. Dielectric relaxations are compared for samples before and after deproteinization, and the protein contamination is found to have a minor effect on the dipole moment of DNA. The effect of a mixed solvent of water and ethanol on the dielectric relaxation of DNA is well interpreted in terms of changes in viscosity and the dielectric constant of the solvent, assuming that the relaxation arises from rotation of the molecule with a quasi‐permanent dipole due to counterion fluctuation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biopolymers Wiley

Dielectric relaxation of DNA solutions. III. Effects of DNA concentration, protein contamination, and mixed solvents

Sakamoto, Masanori; Hayakawa, Reinosuke; Wada, Yasaku
Biopolymers , Volume 18 (11) – Nov 1, 1979
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References (21)

Publisher
Wiley
Copyright
Copyright © 1979 John Wiley & Sons, Inc.
ISSN
0006-3525
eISSN
1097-0282
DOI
10.1002/bip.1979.360181109
pmid
508902
Publisher site
See Article on Publisher Site

Abstract

As a continuation of previous papers (Biopolymers (1976) 15, 879; (1978) 17, 1508), the low‐frequency dielectric relaxation of DNA solutions was studied with a four‐electrode cell and the simultaneous two‐frequency measurement. Below a critical concentration, the dielectric relaxation time agrees with the rotational relaxation time estimated from the reduced viscosity and is almost independent of DNA concentration Cp, and the dielectric increment is proportional to Cp. The critical concentration is approximately 0.02% of DNA for molecular weight Mr 2 × 106 and 0.2% for Mr 4.5 × 105 in 1 mM NaCl. Dielectric relaxations are compared for samples before and after deproteinization, and the protein contamination is found to have a minor effect on the dipole moment of DNA. The effect of a mixed solvent of water and ethanol on the dielectric relaxation of DNA is well interpreted in terms of changes in viscosity and the dielectric constant of the solvent, assuming that the relaxation arises from rotation of the molecule with a quasi‐permanent dipole due to counterion fluctuation.

Journal

Biopolymers – Wiley

Published: Nov 1, 1979

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