Development of immunocytes and immunoglobulin production in long‐term cultures from normal and malignant human lymph nodes

Development of immunocytes and immunoglobulin production in long‐term cultures from normal and... Fifteen human lymph nodes have been grown in vitro. Cells from seven of the nodes eventually developed into apparently permanent lines dominated by cells resembling lymphoblasts. When the results are combined with a previously published series (Pontén, 1967) no difference is found between the frequency at which the phenomenon occurs in lymph nodes obtained from patients without known malignancy (group L), from nodes draining carcinomas (group C), or from malignant lymphomas (group M). It is concluded that the spontaneous establishment of permanent lymphoid cell lines is a characteristic of human lymphoid tissue in general and that no relationship to malignant lymphoma has been proved. A random fibroblastoid growth, apparently unrelated to the appearance of a secondary lymphoid cell production phase, was observed in eight cases, seven of which belonged to group M or C. Five of seven lines were tested repeatedly for globulin production by immunoelectrophoresis and immunoprecipitation combined with autoradiography. Four lines produced immunoglobulins of the IgG type; the fifth, obtained from a tuberculous node, synthesized IgA. Each cell line produced only one detectable molecular species, suggesting a monoclonal origin of the immunoglobulins. The light chains were exclusively of the kappa type, whereas the heavy chains differed with respect to their subgroup specificity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Cancer Wiley

Development of immunocytes and immunoglobulin production in long‐term cultures from normal and malignant human lymph nodes

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Publisher
Wiley
Copyright
Copyright © 1968 Wiley‐Liss, Inc., A Wiley Company
ISSN
0020-7136
eISSN
1097-0215
DOI
10.1002/ijc.2910030203
Publisher site
See Article on Publisher Site

Abstract

Fifteen human lymph nodes have been grown in vitro. Cells from seven of the nodes eventually developed into apparently permanent lines dominated by cells resembling lymphoblasts. When the results are combined with a previously published series (Pontén, 1967) no difference is found between the frequency at which the phenomenon occurs in lymph nodes obtained from patients without known malignancy (group L), from nodes draining carcinomas (group C), or from malignant lymphomas (group M). It is concluded that the spontaneous establishment of permanent lymphoid cell lines is a characteristic of human lymphoid tissue in general and that no relationship to malignant lymphoma has been proved. A random fibroblastoid growth, apparently unrelated to the appearance of a secondary lymphoid cell production phase, was observed in eight cases, seven of which belonged to group M or C. Five of seven lines were tested repeatedly for globulin production by immunoelectrophoresis and immunoprecipitation combined with autoradiography. Four lines produced immunoglobulins of the IgG type; the fifth, obtained from a tuberculous node, synthesized IgA. Each cell line produced only one detectable molecular species, suggesting a monoclonal origin of the immunoglobulins. The light chains were exclusively of the kappa type, whereas the heavy chains differed with respect to their subgroup specificity.

Journal

International Journal of CancerWiley

Published: Mar 15, 1968

References

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