Development of a semi‐continuous two‐stage simultaneous saccharification and fermentation process for enhanced 2,3‐butanediol production by Klebsiella oxytoca

Development of a semi‐continuous two‐stage simultaneous saccharification and fermentation... IntroductionBacterial fermentation can produce 2,3‐butanediol (2,3‐BD) which has received increasing interest due to potential applications in the fuel as well as general bulk industries. 2,3‐BD is considered to be an effective liquid fuel additive because of its high heating value of 27 198 J g−1 (Flickinger ). Also, 2,3‐BD can be converted to 1,3‐butadiene and methyl ethyl ketone by a catalytic dehydration reaction. Both chemicals serve as constituents of synthetic rubbers and as solvents for industrial substances (Garg and Jain ; Celińska and Grajek ; Ji et al. ; Zeng and Sabra ). However, the efficiency of the fermentative 2,3‐BD production process should be further improved to compete with petro‐based products in the market.A number of bacteria, including Klebsiella pneumoniae and Klebsiella oxytoca (Cho et al. ), Enterobacter aerogenes (Jung et al. ), Serratia marcescens (Zhang et al. ), Paenibacillus polymyxa (Gao et al. ), as well as Bacillus amyloliquifaciens (Yang et al. ), produce a relatively large amount of 2,3‐BD from various carbon sources. Among them, K. oxytoca classified into the Risk Group 1, which contains nonpathogenic organisms, in the United States shows a distinctive 2,3‐BD production ability. Accordingly, we have developed a metabolically engineered K. oxytoca strain (K. oxytoca ΔldhA ΔpflB) for the enhanced production of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Letters in Applied Microbiology Wiley

Development of a semi‐continuous two‐stage simultaneous saccharification and fermentation process for enhanced 2,3‐butanediol production by Klebsiella oxytoca

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 The Society for Applied Microbiology
ISSN
0266-8254
eISSN
1472-765X
D.O.I.
10.1111/lam.12845
Publisher site
See Article on Publisher Site

Abstract

IntroductionBacterial fermentation can produce 2,3‐butanediol (2,3‐BD) which has received increasing interest due to potential applications in the fuel as well as general bulk industries. 2,3‐BD is considered to be an effective liquid fuel additive because of its high heating value of 27 198 J g−1 (Flickinger ). Also, 2,3‐BD can be converted to 1,3‐butadiene and methyl ethyl ketone by a catalytic dehydration reaction. Both chemicals serve as constituents of synthetic rubbers and as solvents for industrial substances (Garg and Jain ; Celińska and Grajek ; Ji et al. ; Zeng and Sabra ). However, the efficiency of the fermentative 2,3‐BD production process should be further improved to compete with petro‐based products in the market.A number of bacteria, including Klebsiella pneumoniae and Klebsiella oxytoca (Cho et al. ), Enterobacter aerogenes (Jung et al. ), Serratia marcescens (Zhang et al. ), Paenibacillus polymyxa (Gao et al. ), as well as Bacillus amyloliquifaciens (Yang et al. ), produce a relatively large amount of 2,3‐BD from various carbon sources. Among them, K. oxytoca classified into the Risk Group 1, which contains nonpathogenic organisms, in the United States shows a distinctive 2,3‐BD production ability. Accordingly, we have developed a metabolically engineered K. oxytoca strain (K. oxytoca ΔldhA ΔpflB) for the enhanced production of

Journal

Letters in Applied MicrobiologyWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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