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Determination of the maximum specific uptake capacities for glucose and oxygen in glucose‐limited fed‐batch cultivations of Escherichia coli

Determination of the maximum specific uptake capacities for glucose and oxygen in glucose‐limited... A simple pulse‐based method for the determination of the maximum uptake capacities for glucose and oxygen in glucose limited cultivations of E. coli is presented. The method does not depend on the time‐consuming analysis of glucose or acetate, and therefore can be used to control the feed rate in glucose limited cultivations, such as fed‐batch processes. The application of this method in fed‐batch processes of E. coli showed that the uptake capacity for neither glucose nor oxygen is a constant parameter, as often is assumed in fed‐batch models. The glucose uptake capacity decreased significantly when the specific growth rate decreased below 0.15 h−1 and fell to about 0.6 mmol g−1 h−1 (mmol per g cell dry weight and hour) at the end of fed‐batch fermentations, where μ was approximately 0.02 h−1. The oxygen uptake capacity started to decrease somewhat earlier when μ declined below 0.25 h−1 and was 5 mmol g−1 h−1 at the end of the fermentations. The behavior of both uptake systems is integrated in a dynamic model which allows a better fitting of experimental values for glucose in fed‐batch processes in comparison to generally used unstructured kinetic models. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 347–357, 2001. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Determination of the maximum specific uptake capacities for glucose and oxygen in glucose‐limited fed‐batch cultivations of Escherichia coli

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References (33)

Publisher
Wiley
Copyright
Copyright © 2001 John Wiley & Sons, Inc.
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.1068
Publisher site
See Article on Publisher Site

Abstract

A simple pulse‐based method for the determination of the maximum uptake capacities for glucose and oxygen in glucose limited cultivations of E. coli is presented. The method does not depend on the time‐consuming analysis of glucose or acetate, and therefore can be used to control the feed rate in glucose limited cultivations, such as fed‐batch processes. The application of this method in fed‐batch processes of E. coli showed that the uptake capacity for neither glucose nor oxygen is a constant parameter, as often is assumed in fed‐batch models. The glucose uptake capacity decreased significantly when the specific growth rate decreased below 0.15 h−1 and fell to about 0.6 mmol g−1 h−1 (mmol per g cell dry weight and hour) at the end of fed‐batch fermentations, where μ was approximately 0.02 h−1. The oxygen uptake capacity started to decrease somewhat earlier when μ declined below 0.25 h−1 and was 5 mmol g−1 h−1 at the end of the fermentations. The behavior of both uptake systems is integrated in a dynamic model which allows a better fitting of experimental values for glucose in fed‐batch processes in comparison to generally used unstructured kinetic models. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 73: 347–357, 2001.

Journal

Biotechnology and BioengineeringWiley

Published: Jun 5, 2001

Keywords: fed‐batch‐fermentation; kinetic model; acetate; oxygen uptake; dissolved oxygen tension

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