Detection of the iron complexes with hydrolysis products of cephalexin and cefradine upon high‐performance liquid chromatography/electrospray ionization mass spectrometry analysis

Detection of the iron complexes with hydrolysis products of cephalexin and cefradine upon... INTRODUCTIONHydrolysis of the β‐lactam ring leads to deactivation of β‐lactam antibiotics. It is well known that, in the laboratory, hydrolysis of a β‐lactam ring can be performed in basic solution (the process begins by nucleophilic attack of the hydroxyl anion on the carbonyl carbon atom of the four‐membered β‐lactam ring). In living organisms or in the environment, hydrolysis of the β‐lactam ring is an enzymatic process. There are a number of enzymes (β‐lactamases) which effectively deactivate the antibiotics. Hydrolysis of the β‐lactam ring may be also promoted by metal cations. Because of the importance of the process of hydrolysis of the β‐lactam ring this process has been also theoretically studied in detail.A widely used major group of β‐lactam antibiotics are cephalosporins, e.g. cephalexin (CFL) and cefradine (CFR) (shown in Scheme ).1SchemeStructures of cephalexin (CFL), cefradine (CFR) and their hydrolysis products CFLh, CFRhElectrospray ionization mass spectrometry, usually coupled with high‐performance liquid chromatography (HPLC/ESI‐MS), has been successfully applied to their analysis. However, there is little nuclear magnetic resonance (NMR) data reported concerning the products of their hydrolysis and, to the best of our knowledge, there is no ESI‐MS data reported concerning the products. It is reasonable to expect that the products of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rapid Communications in Mass Spectrometry Wiley

Detection of the iron complexes with hydrolysis products of cephalexin and cefradine upon high‐performance liquid chromatography/electrospray ionization mass spectrometry analysis

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
0951-4198
eISSN
1097-0231
D.O.I.
10.1002/rcm.8073
Publisher site
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Abstract

INTRODUCTIONHydrolysis of the β‐lactam ring leads to deactivation of β‐lactam antibiotics. It is well known that, in the laboratory, hydrolysis of a β‐lactam ring can be performed in basic solution (the process begins by nucleophilic attack of the hydroxyl anion on the carbonyl carbon atom of the four‐membered β‐lactam ring). In living organisms or in the environment, hydrolysis of the β‐lactam ring is an enzymatic process. There are a number of enzymes (β‐lactamases) which effectively deactivate the antibiotics. Hydrolysis of the β‐lactam ring may be also promoted by metal cations. Because of the importance of the process of hydrolysis of the β‐lactam ring this process has been also theoretically studied in detail.A widely used major group of β‐lactam antibiotics are cephalosporins, e.g. cephalexin (CFL) and cefradine (CFR) (shown in Scheme ).1SchemeStructures of cephalexin (CFL), cefradine (CFR) and their hydrolysis products CFLh, CFRhElectrospray ionization mass spectrometry, usually coupled with high‐performance liquid chromatography (HPLC/ESI‐MS), has been successfully applied to their analysis. However, there is little nuclear magnetic resonance (NMR) data reported concerning the products of their hydrolysis and, to the best of our knowledge, there is no ESI‐MS data reported concerning the products. It is reasonable to expect that the products of

Journal

Rapid Communications in Mass SpectrometryWiley

Published: Jan 15, 2018

References

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