Detection of plum pox potyvirus using monoclonal antibodies to structural and non‐structural proteins

Detection of plum pox potyvirus using monoclonal antibodies to structural and non‐structural... Eleven monoclonal antibodies specific to plum pox potyvirus (PPV) coat protein were obtained by hybridoma technology from Spanish PPV isolates. In addition, two monoclonal antibodies specific for PPV cylindrical inclusions (CIP non‐structural proteins) were obtained. The monoclonal antibodies specific for PPV coat protein were assayed by DASI ELISA against 81 PPV isolates. At least nine different epitopes were found and 21 distinct serological patterns of reaction (serogroups) were established using nine selected monoclonal antibodies against the collection of PPV isolates, indicating the high variability of coat protein among PPV isolates. Changes in epitope composition were observed after aphid and mechanical transmission, indicating the occurrence of mixtures of isolates in field trees. Monoclonal antibody 5B reacted with all PPV isolates assayed, with very high affinity, using DASI ELISA. This method was compared with immunocapture‐PCR on field samples in spring, and showed very good coincidence of results. The efficiency of PPV detection can be slightly increased using monoclonal antibodies specific to cylindrical inclusions mixed with monoclonal antibodies against structural proteins, and using mixtures of monoclonal antibodies against different epitopes of coat protein. ELISA‐I and immunoprinting‐ELISA were able to detect CIP and PPV in extracts and tissue section, respectively, of woody plants. Two monoclonal antibodies offer the possibility of distinguishing between Marcus and Dideron PPV types (M or D). These D‐specific monoclonal antibodies can be used in routine tests with high affinity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png EPPO Bulletin Wiley

Detection of plum pox potyvirus using monoclonal antibodies to structural and non‐structural proteins

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0250-8052
eISSN
1365-2338
D.O.I.
10.1111/j.1365-2338.1994.tb01070.x
Publisher site
See Article on Publisher Site

Abstract

Eleven monoclonal antibodies specific to plum pox potyvirus (PPV) coat protein were obtained by hybridoma technology from Spanish PPV isolates. In addition, two monoclonal antibodies specific for PPV cylindrical inclusions (CIP non‐structural proteins) were obtained. The monoclonal antibodies specific for PPV coat protein were assayed by DASI ELISA against 81 PPV isolates. At least nine different epitopes were found and 21 distinct serological patterns of reaction (serogroups) were established using nine selected monoclonal antibodies against the collection of PPV isolates, indicating the high variability of coat protein among PPV isolates. Changes in epitope composition were observed after aphid and mechanical transmission, indicating the occurrence of mixtures of isolates in field trees. Monoclonal antibody 5B reacted with all PPV isolates assayed, with very high affinity, using DASI ELISA. This method was compared with immunocapture‐PCR on field samples in spring, and showed very good coincidence of results. The efficiency of PPV detection can be slightly increased using monoclonal antibodies specific to cylindrical inclusions mixed with monoclonal antibodies against structural proteins, and using mixtures of monoclonal antibodies against different epitopes of coat protein. ELISA‐I and immunoprinting‐ELISA were able to detect CIP and PPV in extracts and tissue section, respectively, of woody plants. Two monoclonal antibodies offer the possibility of distinguishing between Marcus and Dideron PPV types (M or D). These D‐specific monoclonal antibodies can be used in routine tests with high affinity.

Journal

EPPO BulletinWiley

Published: Sep 1, 1994

References

  • Transmission of plum pox potyvirus in Spain
    Avinent, Avinent; Hermoso De Mendoza, Hermoso De Mendoza; Llácer, Llácer
  • Expression and function of potyviral gene products
    Dougherty, Dougherty; Carrington, Carrington
  • Detection of plum pox virus in Spain
    Llácer, Llácer; Cambra, Cambra; Laviña, Laviña

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