Detection of human parvovirus using a molecularly cloned probe

Detection of human parvovirus using a molecularly cloned probe Half of the genomic DNA of the human parvovirus (B19) was cloned in the plasmid pBR322. The cloned DNA was used as a molecular probe for the detection of parvovirus in serum by means of a dot hybridization test. In an assay of 26 samples, the dot hybridization test was found to be of comparable sensitivity and to be as rapid as radioimmunoassay for viral antigen detection; it is potentially useful as a diagnostic test. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

Detection of human parvovirus using a molecularly cloned probe

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Abstract

Half of the genomic DNA of the human parvovirus (B19) was cloned in the plasmid pBR322. The cloned DNA was used as a molecular probe for the detection of parvovirus in serum by means of a dot hybridization test. In an assay of 26 samples, the dot hybridization test was found to be of comparable sensitivity and to be as rapid as radioimmunoassay for viral antigen detection; it is potentially useful as a diagnostic test.

Journal

Journal of Medical VirologyWiley

Published: Jun 1, 1985

References

  • Episomal DNA of a BK virus variant in a human insulinoma
    Caputo, Caputo; Corallini, Corallini; Grossi, Grossi; Carra, Carra; Balboni, Balboni; Negrini, Negrini; Milanesi, Milanesi; Federspil, Federspil; Barbanti‐Brodano, Barbanti‐Brodano
  • Aplastic crises in haemolytic anaemias: The role of a parvovirus‐like agent
    Davis, Davis
  • Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: Comparison with results for other viral markers
    Scotto, Scotto; Hadchouel, Hadchouel; Hery, Hery; Yvart, Yvart; Tiollais, Tiollais; Brechot, Brechot

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