Detection of hepatitis C virus (HCV) RNA by PCR related to HCV antibodies in serum and liver histology in swedish blood donors

Detection of hepatitis C virus (HCV) RNA by PCR related to HCV antibodies in serum and liver... Zhi-Bing Yun, Gudrun Lindh, Ola Weiland, Bo Johansson, and Anders Sonnerborg Department of Virology, the Central Microbiological Laboratory of Stockholm County Council (Z.-B.Y.,B.J., A S . ) and Department of Infectious Diseases, Huddinge Hospital, Karolinska Institute (G.L., 0.W., A S . ) , Stockholm, Sweden Serum samples from 103 blood donors or patients with slightly increased serum levels of liver enzymes were tested for antibodies t o hepatitis C virus (anti-HCV) using second generation tests and for HCV RNA by the polymerase chain reaction (PCR). PCR was in a nested configuration, using primer pairs from the 5'-nontranslated region. The anti-HCV antibody was found by enzyme linked immunosorbent assay (ELISA) in 93 patients. The anti-HCV confirmatory second generation recombinant immunoblot assay (RIBA) was positive in 44, indeterminate in 34 and negative in 25 subjects. Histopathological examination of the liver was carried out i n 51 subjects. HCV RNA was detected in serum of 39/41 (95%) RlBA positive patients, and in 7/34 (21%) RlBA indeterminate subjects, but in none of the RlBA negative subjects. All but one of the PCR positive patients with a RlBA indeterminate pattern exhibited the C22 band. HCV RNA was found in the serum of all http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

Detection of hepatitis C virus (HCV) RNA by PCR related to HCV antibodies in serum and liver histology in swedish blood donors

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Abstract

Zhi-Bing Yun, Gudrun Lindh, Ola Weiland, Bo Johansson, and Anders Sonnerborg Department of Virology, the Central Microbiological Laboratory of Stockholm County Council (Z.-B.Y.,B.J., A S . ) and Department of Infectious Diseases, Huddinge Hospital, Karolinska Institute (G.L., 0.W., A S . ) , Stockholm, Sweden Serum samples from 103 blood donors or patients with slightly increased serum levels of liver enzymes were tested for antibodies t o hepatitis C virus (anti-HCV) using second generation tests and for HCV RNA by the polymerase chain reaction (PCR). PCR was in a nested configuration, using primer pairs from the 5'-nontranslated region. The anti-HCV antibody was found by enzyme linked immunosorbent assay (ELISA) in 93 patients. The anti-HCV confirmatory second generation recombinant immunoblot assay (RIBA) was positive in 44, indeterminate in 34 and negative in 25 subjects. Histopathological examination of the liver was carried out i n 51 subjects. HCV RNA was detected in serum of 39/41 (95%) RlBA positive patients, and in 7/34 (21%) RlBA indeterminate subjects, but in none of the RlBA negative subjects. All but one of the PCR positive patients with a RlBA indeterminate pattern exhibited the C22 band. HCV RNA was found in the serum of all

Journal

Journal of Medical VirologyWiley

Published: Jan 1, 1993

References

  • Molecular biology of the hepatitis C viruses: Implications for diagnosis, development and control of viral disease
    Houghton, Houghton; Weiner, Weiner; Han, Han; Kuo, Kuo; Choo, Choo

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