Detection and quantification of melatonin in a dinoflagellate, Gonyaulax polyedra : Solutions to the problem of methoxyindole destruction in non‐vertebrate material

Detection and quantification of melatonin in a dinoflagellate, Gonyaulax polyedra : Solutions to... Poeggeler B, Hardeland R. Detection and quantification of melatonin in a dinoflagellate, Gonyaulax polyedra: Solutions to the problem of methoxyindole destruction in non‐vertebrate material. J. Pineal Res. 1994: 17: 1–10. Preservative procedures are described for the extraction and quantification of melatonin in cell material from the dinoflagellate Gonyaulax polyedra, an organism in which this indoleamine is rapidly degraded due to interaction with free oxygen radicals and photooxida‐tion. Cells were shock‐frozen in liquid nitrogen and pulverized. Various extraction methods were applied to the powder, and rates of recovery were compared. For the determination of melatonin by high performance liquid chromatography (HPLC), extractions with acetone or perchloric acid were more suitable than the use of other solvents. For purposes of radioimmunoassay (RIA), extraction with acetone gave the best results. Several other inorganic solvents, which are often applied in melatonin research, such as chloroform, dichloromethane, and diethyl ether, led to considerable losses of the indoleamine. The procedures developed for HPLC with either electrochemical or fluorescence detection also allow the quantification of other indolic compounds, in particular, tryptophan and 5‐methoxytryptamine. The methods described may be of value in the further search for melatonin and related indoleamines in non‐vertebrate material, especially, from unicells, multicellular plants, and invertebrates. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Pineal Research Wiley

Detection and quantification of melatonin in a dinoflagellate, Gonyaulax polyedra : Solutions to the problem of methoxyindole destruction in non‐vertebrate material

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Publisher
Wiley
Copyright
Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0742-3098
eISSN
1600-079X
DOI
10.1111/j.1600-079X.1994.tb00106.x
Publisher site
See Article on Publisher Site

Abstract

Poeggeler B, Hardeland R. Detection and quantification of melatonin in a dinoflagellate, Gonyaulax polyedra: Solutions to the problem of methoxyindole destruction in non‐vertebrate material. J. Pineal Res. 1994: 17: 1–10. Preservative procedures are described for the extraction and quantification of melatonin in cell material from the dinoflagellate Gonyaulax polyedra, an organism in which this indoleamine is rapidly degraded due to interaction with free oxygen radicals and photooxida‐tion. Cells were shock‐frozen in liquid nitrogen and pulverized. Various extraction methods were applied to the powder, and rates of recovery were compared. For the determination of melatonin by high performance liquid chromatography (HPLC), extractions with acetone or perchloric acid were more suitable than the use of other solvents. For purposes of radioimmunoassay (RIA), extraction with acetone gave the best results. Several other inorganic solvents, which are often applied in melatonin research, such as chloroform, dichloromethane, and diethyl ether, led to considerable losses of the indoleamine. The procedures developed for HPLC with either electrochemical or fluorescence detection also allow the quantification of other indolic compounds, in particular, tryptophan and 5‐methoxytryptamine. The methods described may be of value in the further search for melatonin and related indoleamines in non‐vertebrate material, especially, from unicells, multicellular plants, and invertebrates.

Journal

Journal of Pineal ResearchWiley

Published: Aug 1, 1994

References

  • Potential radioprotective agents, 1. Homologs of melatonin
    BLICKENSTAFF, BLICKENSTAFF; BRANDSTADTER, BRANDSTADTER; REDDY, REDDY; WITT, WITT
  • Melatonin biosynthesis in Drosophila: Its nature and effects
    FINOCCHIARO, FINOCCHIARO; CALLEBERT, CALLEBERT; LAUNAY, LAUNAY; JALLON, JALLON
  • Pineal hormone melatonin oscillates also in the dinoflagellate Gonyaulax polyedra
    POEGGELER, POEGGELER; BALZER, BALZER; HARDELAND, HARDELAND; LERCHL, LERCHL
  • A versatile homogenous one‐tube scintillation proximity radioimmunoassay for melatonin
    POEGGELER, POEGGELER; HUETHER, HUETHER
  • Melatonin, hydroxyl radical‐mediated oxidative damage and aging: A hypothesis
    POEGGELER, POEGGELER; REITER, REITER; TAN, TAN; CHEN, CHEN; MANCHESTER, MANCHESTER

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