Detection and clinical features of hepatitis C virus type 6 infections in blood donors from Hong Kong

Detection and clinical features of hepatitis C virus type 6 infections in blood donors from Hong... The genotype distribution of hepatitis C virus (HCV) was investigated in 212 viraemic blood donors from Hong Kong. A subset of the samples was investigated using three different genotyping assays to establish the accuracy of each in this population. These assays were restriction fragment length polymorphism (RFLP) of amplified 5′ noncoding region (5′NCR) sequences, RFLP of the core region, and a serotyping assay using peptides from two antigenic regions of NS4. Genotypes detected in Hong Kong blood donors were 1a (6.2%), 1b (58.8%), 2a (1.4%), 2b (1.4%), 3a (1.9%), and 6a (27.0%). All genotyping assays produced concordant results. No evidence was obtained for the presence of type 6 group variants recently identified in Southeast Asia, other than type 6a. A serotyping assay based upon the detection of type‐specific antibody to epitopes in NS4 produced similar results to the genotyping assays (98% concordance), but a reduced sensitivity (75%) compared with genotyping methods. Sequence variation in NS4 was not the cause of the reduced rate of detection of type 6 antibody in this population. Eighty‐four percent donors infected with type 6a were male, compared to 75% donors infected with type 1b. The median alanine transaminase (ALT) level in type 6 infected donors was lower than in type 1b, (43.8 and 51.1 U/l, respectively) although these values were not statistically significant (P = 0.094). There was no significant difference between the ages of donors infected with types 1b and 6a. Risk factors for HCV infection in the blood donors included blood transfusion, intravenous drug abuse, and tattooing. A significantly greater number of donors infected with HCV‐6a reported a history of drug abuse (66%) than donors infected with HCV‐1b (7%). © 1996 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

Detection and clinical features of hepatitis C virus type 6 infections in blood donors from Hong Kong

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 1996 Wiley‐Liss, Inc.
ISSN
0146-6615
eISSN
1096-9071
D.O.I.
10.1002/(SICI)1096-9071(199610)50:2<168::AID-JMV10>3.0.CO;2-I
Publisher site
See Article on Publisher Site

Abstract

The genotype distribution of hepatitis C virus (HCV) was investigated in 212 viraemic blood donors from Hong Kong. A subset of the samples was investigated using three different genotyping assays to establish the accuracy of each in this population. These assays were restriction fragment length polymorphism (RFLP) of amplified 5′ noncoding region (5′NCR) sequences, RFLP of the core region, and a serotyping assay using peptides from two antigenic regions of NS4. Genotypes detected in Hong Kong blood donors were 1a (6.2%), 1b (58.8%), 2a (1.4%), 2b (1.4%), 3a (1.9%), and 6a (27.0%). All genotyping assays produced concordant results. No evidence was obtained for the presence of type 6 group variants recently identified in Southeast Asia, other than type 6a. A serotyping assay based upon the detection of type‐specific antibody to epitopes in NS4 produced similar results to the genotyping assays (98% concordance), but a reduced sensitivity (75%) compared with genotyping methods. Sequence variation in NS4 was not the cause of the reduced rate of detection of type 6 antibody in this population. Eighty‐four percent donors infected with type 6a were male, compared to 75% donors infected with type 1b. The median alanine transaminase (ALT) level in type 6 infected donors was lower than in type 1b, (43.8 and 51.1 U/l, respectively) although these values were not statistically significant (P = 0.094). There was no significant difference between the ages of donors infected with types 1b and 6a. Risk factors for HCV infection in the blood donors included blood transfusion, intravenous drug abuse, and tattooing. A significantly greater number of donors infected with HCV‐6a reported a history of drug abuse (66%) than donors infected with HCV‐1b (7%). © 1996 Wiley‐Liss, Inc.

Journal

Journal of Medical VirologyWiley

Published: Oct 1, 1996

References

  • Influence of viraemia and genotype upon serological reactivity in screening assays for antibody to hepatitis C virus
    Dhaliwal, Dhaliwal; Prescott, Prescott; Dow, Dow; Davidson, Davidson; Brown, Brown; Yap, Yap; Follett, Follett; Simmonds, Simmonds
  • Genotypes and titers of hepatitis C virus for predicting response to interferon in patients with chronic hepatitis C
    Hino, Hino; Sainokami, Sainokami; Shimoda, Shimoda; Iino, Iino; Wang, Wang; Okamoto, Okamoto; Miyakawa, Miyakawa; Mayumi, Mayumi
  • Genotypic analysis of hepatitis C virus in American patients
    Mahaney, Mahaney; Tedeschi, Tedeschi; Maertens, Maertens; Dibisceglie, Dibisceglie; Vergalla, Vergalla; Hoofnagle, Hoofnagle; Sallie, Sallie
  • Detection of three types of hepatitis C virus in blood donors: Investigation of type‐specific differences in serological reactivity and rate of alanine aminotransferase abnormalities
    McOmish, McOmish; Chan, Chan; Dow, Dow; Gillon, Gillon; Frame, Frame; Crawford, Crawford; Yap, Yap; Follett, Follett; Simmonds, Simmonds
  • Evaluation of a multiple peptide assay for typing of antibodies to the hepatitis C virus: Relation to genomic typing by the polymerase chain reaction
    Zhang, Zhang; Yun, Yun; Chen, Chen; Sonnerborg, Sonnerborg; Sallberg, Sallberg

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