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KINETIC STUDY OF DEFINED METHANE FERMENTATION OF LACTOSE -
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- Publisher
- Wiley
- Copyright
- Copyright © 1988 John Wiley & Sons, Inc.
- ISSN
- 0006-3592
- eISSN
- 1097-0290
- DOI
- 10.1002/bit.260320106
- pmid
- 18584715
- Publisher site
- See Article on Publisher Site
Abstract
10.1002/bit.260320106.abs The defined microbial cultures for methane generation from lactose were investigated. A mixed culture consisting of homolactic (Streptococcus lactis), homoacetic (Clostridium formicoaceticum), and acetate‐utilizing methanogenic (Methanococcus mazei) bacteria was used to convert lactose and whey permeate to methane at mesophilic temperatures (35–37°C) and a pH around 7.0. Lactose was first converted to lactic acid by S. lactis, then to acetic acid by C. formicoaceticum, and finally to methane and CO2 by M. mazei. About 5.3 mol methane were obtained from each mole of lactose consumed, and the conversion of acetate to methane was the rate‐limiting step for this mixed‐culture fermentation.
Journal
Biotechnology and Bioengineering – Wiley
Published: Jun 20, 1988