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F. Lahnsteiner, B. Berger, Á. Horváth, B. Urbányi (2004)
Studies on the semen biology and sperm cryopreservation in the sterlet, Acipenser ruthenus L.Aquaculture Research, 35
R. Billard, J. Cosson, S. Noveiri, M. Pourkazemi (2004)
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H. Jähnichen, D. Wamecke, E. Trölsch, K. Kohlmann, H. Bergler, H. Pluta (1999)
Motility and fertilizing capability of cryopreserved Acipenser ruthenus L. spermJournal of Applied Ichthyology, 15
M. Pšenička, G. Dietrich, M. Wojtczak, J. Nynca, M. Rodina, O. Linhart, J. Cosson, A. Ciereszko (2008)
Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO.Cryobiology, 56 3
M. Rodina, T. Policar, O. Linhart, C. Rougeot (2008)
Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis)Journal of Applied Ichthyology, 24
J. Glogowski, R. Kolman, M. Szczepkowski, Á. Horváth, B. Urbányi, P. Sieczyński, A. Rzemieniecki, J. Domagała, W. Demianowicz, R. Kowalski, A. Ciereszko (2002)
Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanolAquaculture, 211
Á. Horváth, W. Wayman, B. Urbányi, K. Ware, J. Dean, T. Tiersch (2005)
The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon speciesAquaculture, 247
R. Billard, G. Lecointre (2000)
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Summary The present study examined the effectiveness of different cryoprotectan uses for cryopreservation of sterlet (Acipenser ruthenus) sperm. Four different cryoprotectans (dimethyl‐sulfoxide (DMSO), dimethylacetamide (DMA), ethyleneglycol (EG) and methanol (MET)) in concentrations of 5 and 10% in the extender media (30 mm sucrose, 1 mm KCl, 25 mm Tris–HCl pH 8.5) were used for the cryopreservation of sperm from five sterlet males. Percentages of motility, velocity, fertilization and hatching rate were measured. The highest post‐thawing motility was observed in sperm samples cryopreserved with 10% MET (46 ± 19%), 10% DMA (47 ± 18%) and 10% DMSO (45 ± 7%). Fertilization rate in the control (fresh sperm) was 69 ± 9% and the hatching rate 61 ± 8%. A higher hatching rate after cryopreservation was obtained with 10% MET (32 ± 17%) and 5% DMA (23 ± 3%) when compared to data obtained with 10% DMA (9 ± 5%), 5% MET and 5 and 10% DMSO, wherein the hatching rate was around zero. Our results demonstrate that DMA can be used for cryopreservation of sturgeon spermatozoa.
Journal of Applied Ichthyology – Wiley
Published: Oct 1, 2011
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