Cryopreservation of Platelets Isolated with the IBM 2997 Blood Cell Separator: A Rapid and Simplified Approach

Cryopreservation of Platelets Isolated with the IBM 2997 Blood Cell Separator: A Rapid and... The equivalent of 5–7 units of platelets, isolated from a single donor with the IBM Blood Cell Processor 2997 using a dual stage separation chamber, was frozen with the cryoprotectant dimethylsulfoxide (DMSO). The DMSO‐saline solution was added directly to the platelets, and the platelets were frozen in a polyvinyl chloride plastic bag by storage in a ‐80°C mechanical freezer. Washing the thawed platelets with a phosphate‐buffered sodium chloride‐dextrose solution, pH of 5.0, removed about 95% of the DMSO. In vitro freeze‐thaw‐wash recovery was 80%, and in vivo 51Cr platelet recovery was 31%. Platelet dense body granules were well maintained after freezing, thawing, and washing. This is a safe and effective method of platelet cryopreservation which can be performed in less time than other currently used methods. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Vox Sanguinis Wiley

Cryopreservation of Platelets Isolated with the IBM 2997 Blood Cell Separator: A Rapid and Simplified Approach

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Publisher
Wiley
Copyright
© 1982 Blackwell Publishing Ltd
ISSN
0042-9007
eISSN
1423-0410
D.O.I.
10.1111/j.1423-0410.1982.tb00030.x
Publisher site
See Article on Publisher Site

Abstract

The equivalent of 5–7 units of platelets, isolated from a single donor with the IBM Blood Cell Processor 2997 using a dual stage separation chamber, was frozen with the cryoprotectant dimethylsulfoxide (DMSO). The DMSO‐saline solution was added directly to the platelets, and the platelets were frozen in a polyvinyl chloride plastic bag by storage in a ‐80°C mechanical freezer. Washing the thawed platelets with a phosphate‐buffered sodium chloride‐dextrose solution, pH of 5.0, removed about 95% of the DMSO. In vitro freeze‐thaw‐wash recovery was 80%, and in vivo 51Cr platelet recovery was 31%. Platelet dense body granules were well maintained after freezing, thawing, and washing. This is a safe and effective method of platelet cryopreservation which can be performed in less time than other currently used methods.

Journal

Vox SanguinisWiley

Published: Dec 1, 1982

References

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