Oligonucleosomal fragmentation of chromosomes in dying cells is a hallmark of apoptosis. Little is known about how it is executed or what cellular components are involved. We show that crn‐1, a Caenorhabditis elegans homologue of human flap endonuclease‐1 (FEN‐1) that is normally involved in DNA replication and repair, is also important for apoptosis. Reduction of crn‐1 activity by RNA interference resulted in cell death phenotypes similar to those displayed by a mutant lacking the mitochondrial endonuclease CPS‐6/endonuclease G. CRN‐1 localizes to nuclei and can associate and cooperate with CPS‐6 to promote stepwise DNA fragmentation, utilizing the endonuclease activity of CPS‐6 and both the 5′–3′ exonuclease activity and a previously uncharacterized gap‐dependent endonuclease activity of CRN‐1. Our results suggest that CRN‐1/FEN‐1 may play a critical role in switching the state of cells from DNA replication/repair to DNA degradation during apoptosis.
The EMBO Journal – Wiley
Published: Jul 1, 2003
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera