Crk is required for apoptosis in Xenopus egg extracts

Crk is required for apoptosis in Xenopus egg extracts Apoptosis is essential for the development and homeostasis of multicellular organisms. Recently, a cell‐free extract prepared from Xenopus eggs was shown to recapitulate intracellular apoptotic pathways in vitro. While many stimuli have been shown to trigger apoptosis in a variety of cell types, the intracellular signaling pathways involved in apoptosis remain largely unknown. Here we show that addition of a recombinant protein containing the phosphotyrosine binding (SH2) domain from the adaptor protein crk, but not those derived from a panel of other signaling proteins, can prevent apoptosis in the Xenopus egg extract system. Furthermore, immunodepletion of endogenous crk protein from the egg extracts, or addition of anti‐crk antisera to these extracts, prevents apoptosis. The ability to undergo apoptosis can be restored to these extracts by addition of recombinant crk protein. These results directly demonstrate that crk participates in apoptotic signaling. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

Crk is required for apoptosis in Xenopus egg extracts

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Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
DOI
10.1093/emboj/16.2.230
Publisher site
See Article on Publisher Site

Abstract

Apoptosis is essential for the development and homeostasis of multicellular organisms. Recently, a cell‐free extract prepared from Xenopus eggs was shown to recapitulate intracellular apoptotic pathways in vitro. While many stimuli have been shown to trigger apoptosis in a variety of cell types, the intracellular signaling pathways involved in apoptosis remain largely unknown. Here we show that addition of a recombinant protein containing the phosphotyrosine binding (SH2) domain from the adaptor protein crk, but not those derived from a panel of other signaling proteins, can prevent apoptosis in the Xenopus egg extract system. Furthermore, immunodepletion of endogenous crk protein from the egg extracts, or addition of anti‐crk antisera to these extracts, prevents apoptosis. The ability to undergo apoptosis can be restored to these extracts by addition of recombinant crk protein. These results directly demonstrate that crk participates in apoptotic signaling.

Journal

The EMBO JournalWiley

Published: Mar 15, 1998

Keywords: ; ; ;

References

  • Cell death in health and disease: the biology and regulation of apoptosis
    Bellamy, COC; Malcomson, RDG; Harrison, DJ; Wyllie, AH
  • The cell‐death machine
    Chinnaiyan, AM; Dixit, VM
  • Bcl‐2 regulates activation of apoptotic proteases in a cell‐free system
    Cosulich, SC; Green, G; Clarke, PR
  • Mechanisms and functions of cell death
    Ellis, RE; Yuan, J; Horvitz, HR
  • Control of adhesion‐dependent cell survival by focal adhesion kinase
    Frisch, SM; Vuori, K; Ruoslahti, E; Chan‐Hui, P‐Y
  • R‐ras promotes apoptosis caused by growth factor deprivation via a Bcl‐2 suppressible mechanism
    Wang, H; Millan, J; Cox, A; Der, C; Rapp, U; Beck, T; Zha, H; Reed, J

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