Access the full text.
Sign up today, get DeepDyve free for 14 days.
G. Ruyter, P. Postma, K. Dam (1991)
Control of glucose metabolism by enzyme IIGlc of the phosphoenolpyruvate-dependent phosphotransferase system in Escherichia coliJournal of Bacteriology, 173
W. Barnell, K. Yi, Tyrrell Conway (1990)
Sequence and genetic organization of a Zymomonas mobilis gene cluster that encodes several enzymes of glucose metabolismJournal of Bacteriology, 172
D. Kell, H. Westerhoff (1986)
Towards a rational approach to the optimization of flux in microbial biotransformationsTrends in Biotechnology, 4
H. Westerhoff, K. Dam (1987)
Thermodynamics and Control of Biological Free-Energy Transduction
James Russell, Gregory Cook (1995)
Energetics of bacterial growth: balance of anabolic and catabolic reactions.Microbiological reviews, 59 1
E. Algar, R. Scopes (1985)
Studies on cell-free metabolism: Ethanol production by extracts of Zymomonas mobilisJournal of Biotechnology, 2
J. Snoep, L. Yomano, H. Westerhoff, L. Ingram (1995)
Protein burden in Zymomonas mobilis: negative flux and growth control due to overproduction of glycolytic enzymesMicrobiology, 141
Fermentationt, Y. Osman, T. Conway, S. Bonetti, L., 0., Ingram (1987)
Glycolytic flux in Zymomonas mobilis: enzyme and metabolite levels during batch fermentationJournal of Bacteriology, 169
L. Bisson, D. Coons, A. Kruckeberg, D. Lewis (1993)
Yeast sugar transporters.Critical reviews in biochemistry and molecular biology, 28 4
M. Navas, S. Cerdán, J. Gancedo (1993)
Futile cycles in Saccharomyces cerevisiae strains expressing the gluconeogenic enzymes during growth on glucose.Proceedings of the National Academy of Sciences of the United States of America, 90
L. Yomano, R. Scopes, L., 0., '. Ingram (1993)
Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coliJournal of Bacteriology, 175
S. Egan, R. Fliege, Suxiang Tong, Annemarie Shibata, R. Wolf, Tyrrell Conway (1992)
Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operonJournal of Bacteriology, 174
Kevin Barrow, J. Collins, Raymond Norton, P. Rogers, G. Smith (1984)
31P nuclear magnetic resonance studies of the fermentation of glucose to ethanol by Zymomonas mobilis.The Journal of biological chemistry, 259 9
R. Simon, U. Priefer, A. Pühler (1983)
A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative BacteriaBio/Technology, 1
H. Westerhoff, K. Hellingwerf, K. Dam (1983)
Thermodynamic efficiency of microbial growth is low but optimal for maximal growth rate.Proceedings of the National Academy of Sciences of the United States of America, 80 1
J. Galazzo, J. Bailey (1990)
Fermentation pathway kinetics and metabolic flux control in suspended and immobilized Saccharomyces cerevisiaeEnzyme and Microbial Technology, 12
H Aldrich, L. McDowell, M Barbosa, L. Yomano, R. Scopes, L. Ingram (1992)
Immunocytochemical localization of glycolytic and fermentative enzymes in Zymomonas mobilisJournal of Bacteriology, 174
W. Barnell, J. Liu, T Hesman, M O'Neill, T. Conway (1992)
The Zymomonas mobilis glf, zwf, edd, and glk genes form an operon: localization of the promoter and identification of a conserved sequence in the regulatory regionJournal of Bacteriology, 174
Haejung An, R. Scopes, Marcos Rodríguez, K. Keshav, L. Ingram (1991)
Gel electrophoretic analysis of Zymomonas mobilis glycolytic and fermentative enzymes: identification of alcohol dehydrogenase II as a stress proteinJournal of Bacteriology, 173
M. Bradford (1976)
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Analytical biochemistry, 72
I. Schaaff, J. Heinisch, F. Zimmermann (1989)
Overproduction of glycolytic enzymes in yeastYeast, 5
L. Viikari, D. Berry (1988)
CARBOHYDRATE METABOLISM IN ZYMOMONASCritical Reviews in Biotechnology, 7
Alberto Sols (1981)
Multimodulation of enzyme activity.Current topics in cellular regulation, 19
S. Curtis, W. Epstein (1975)
Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinaseJournal of Bacteriology, 122
R. Simon, M. O'Connell, M. Labes, A. Pühler (1986)
Plasmid vectors for the genetic analysis and manipulation of rhizobia and other gram-negative bacteria.Methods in enzymology, 118
N. Arfman, V. Worrell, L. Ingram (1992)
Use of the tac promoter and lacIq for the controlled expression of Zymomonas mobilis fermentative genes in Escherichia coli and Zymomonas mobilisJournal of Bacteriology, 174
Min Zhang, C. Eddy, K. Deanda, M. Finkelstein, S. Picataggio (1995)
Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilisScience, 267
Peter Jensen, O. Michelsen, Hans Westerhoff (1993)
Control analysis of the dependence of Escherichia coli physiology on the H(+)-ATPase.Proceedings of the National Academy of Sciences of the United States of America, 90 17
R. Eisenberg, W. Dobrogosz (1967)
Gluconate Metabolism in Escherichia coliJournal of Bacteriology, 93
J. Snoep, N. Arfman, L. Yomano, R. Fliege, T. Conway, L. Ingram (1994)
Reconstruction of glucose uptake and phosphorylation in a glucose-negative mutant of Escherichia coli by using Zymomonas mobilis genes encoding the glucose facilitator protein and glucokinaseJournal of Bacteriology, 176
J. Liu, W. Barnell, Tyrrell Conway (1992)
The polycistronic mRNA of the Zymomonas mobilis glf-zwf-edd-glk operon is subject to complex transcript processingJournal of Bacteriology, 174
R. Scopes, V. Testolin, A. Stoter, K. Griffiths-Smith, E. Algar (1985)
Simultaneous purification and characterization of glucokinase, fructokinase and glucose-6-phosphate dehydrogenase from Zymomonas mobilis.The Biochemical journal, 228 3
10.1002/(SICI)1097-0290(19960720)51:2<190::AID-BIT8>3.3.CO;2-L Glycolytic genes in Zymomonas mobilis are highly expressed and constitute half of the cytoplasmic protein. The first four genes (glf, zwf, edd, glk) in this pathway form an operon encoding a glucose permease, glucose 6‐phosphate dehydrogenase (G6‐P dehydrogenase), 6‐phosphogluconate dehydratase, and glucokinase, respectively. Each gene was overexpressed from a tac promoter to investigate the control of glycolysis during the early stages of batch fermentation when flux (qCO2) is highest. Almost half of flux control appears to reside with G6‐P dehydrogenase (CJG6‐P dehydrogenase = 0.4). Although Z. mobilis exhibits one of the highest rates of glycolysis known, recombinants with elevated G6‐P dehydrogenase had a 10% to 13% higher glycolytic flux than the native organism. A small increase in flux was also observed for recombinants expressing glf. Results obtained did not allow a critical evaluation of glucokinase and this enzyme may also represent an important control point. 6‐Phosphogluconate dehydratase appears to be saturating at native levels. With constructs containing the full operon, growth rate and flux were both reduced, complicating interpretations. However, results obtained were also consistent with G6‐P dehydrogenase as a primary site of control. Flux was 17% higher in operon constructs which exhibited a 17% increase in G6‐P dehydrogenase specific activity, relative to the average of other operon constructs which contain a frameshift mutation in zwf. It is unlikely that all flux control residues solely in G6‐P dehydrogenase (calculated CJG6‐P dehydrogenase = 1.0) although these results further support the importance of this enzyme. As reported in previous studies, changes in flux were not accompanied by changes in growth rate providing further evidence that ATP production does not limit biosynthesis in rich complex medium. © 1996 John Wiley & Sons, Inc.
Biotechnology and Bioengineering – Wiley
Published: Jul 20, 1996
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.