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Continuous conversion of starch to glucose with immobilized glucoamylase

Continuous conversion of starch to glucose with immobilized glucoamylase Partially purified glucoamylase from Aspergillus awamori NRRL 3112 was immobilized on diethylaminoethyl cellulose in the presence of low ionic‐strength acetate buffers at pH 4.2. The active enzyme–cellulose complex was used to convert starch substrates continuously to glucose in stirred reactors. Substrate concentrations as high as 30% could be quantitatively converted to glucose at a rate of more than 25 mg/min/liter at 55°C for periods of 3 to 4 weeks in a 4‐liter reactor. Shutdowns were due to mechanical problems and not to loss of enzymes, which could be recovered with no appreciable loss of specific activity. Transfer products, such as isomaltose and panose, were present in immobilized enzyme‐produced syrups but to no greater degree than in soluble glucoamylase digests of starch. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Continuous conversion of starch to glucose with immobilized glucoamylase

Biotechnology and Bioengineering , Volume 13 (2) – Mar 1, 1971

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References (4)

Publisher
Wiley
Copyright
Copyright © 1971 John Wiley & Sons, Inc.
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.260130211
pmid
5580687
Publisher site
See Article on Publisher Site

Abstract

Partially purified glucoamylase from Aspergillus awamori NRRL 3112 was immobilized on diethylaminoethyl cellulose in the presence of low ionic‐strength acetate buffers at pH 4.2. The active enzyme–cellulose complex was used to convert starch substrates continuously to glucose in stirred reactors. Substrate concentrations as high as 30% could be quantitatively converted to glucose at a rate of more than 25 mg/min/liter at 55°C for periods of 3 to 4 weeks in a 4‐liter reactor. Shutdowns were due to mechanical problems and not to loss of enzymes, which could be recovered with no appreciable loss of specific activity. Transfer products, such as isomaltose and panose, were present in immobilized enzyme‐produced syrups but to no greater degree than in soluble glucoamylase digests of starch.

Journal

Biotechnology and BioengineeringWiley

Published: Mar 1, 1971

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