Conditioned‐fear stress increases Fos expression in monoaminergic and GABAergic neurons of the locus coeruleus and dorsal raphe nuclei

Conditioned‐fear stress increases Fos expression in monoaminergic and GABAergic neurons of the... Many studies have demonstrated that physical or psychological stress can increase Fos expression in brainstem monoaminergic nuclei. Little is known, however, about the extent to which stress increases the expression of Fos in monoaminergic and nonmonoaminergic neurons in the brainstem. We examined the effects of conditioned‐fear (CF) stress following mild footshock (FS) as unconditioned stress on Fos expression in the monoaminergic and GABAergic neurons of the ventral tegmental area (VTA), locus coeruleus (LC), and dorsal raphe nucleus (DR) in rats. The CF stress significantly increased the number of Fos‐positive (Fos+) cells in both the LC and DR, whereas it did not increase the number in the VTA. Using a double‐labeling technique, we combined Fos immunostaining with that for tyrosine hydroxylase (TH), serotonin (5‐HT), or GABA for histochemical identification of the CF stress‐induced Fos+ neurons. The percentage of TH/Fos double‐labeled cells resulting from CF stress was 63% of the Fos+ cells in the LC, whereas 52% of the Fos+ cells contained 5‐HT in the DR. We also found that approximately 60% of the CF stress‐induced Fos+ cells were GABAergic neurons in these brain regions. These results indicate that CF stress induces intense Fos expression in the noradrenergic LC and serotonergic DR neurons, but not in the dopaminergic VTA neurons. They also indicate that not only monoaminergic neurons but also GABAergic neurons within the LC and DR are activated by the stress. Synapse 45:46–51, 2002. © 2002 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Synapse Wiley

Conditioned‐fear stress increases Fos expression in monoaminergic and GABAergic neurons of the locus coeruleus and dorsal raphe nuclei

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Publisher
Wiley
Copyright
Copyright © 2002 Wiley‐Liss, Inc.
ISSN
0887-4476
eISSN
1098-2396
DOI
10.1002/syn.10086
pmid
12112413
Publisher site
See Article on Publisher Site

Abstract

Many studies have demonstrated that physical or psychological stress can increase Fos expression in brainstem monoaminergic nuclei. Little is known, however, about the extent to which stress increases the expression of Fos in monoaminergic and nonmonoaminergic neurons in the brainstem. We examined the effects of conditioned‐fear (CF) stress following mild footshock (FS) as unconditioned stress on Fos expression in the monoaminergic and GABAergic neurons of the ventral tegmental area (VTA), locus coeruleus (LC), and dorsal raphe nucleus (DR) in rats. The CF stress significantly increased the number of Fos‐positive (Fos+) cells in both the LC and DR, whereas it did not increase the number in the VTA. Using a double‐labeling technique, we combined Fos immunostaining with that for tyrosine hydroxylase (TH), serotonin (5‐HT), or GABA for histochemical identification of the CF stress‐induced Fos+ neurons. The percentage of TH/Fos double‐labeled cells resulting from CF stress was 63% of the Fos+ cells in the LC, whereas 52% of the Fos+ cells contained 5‐HT in the DR. We also found that approximately 60% of the CF stress‐induced Fos+ cells were GABAergic neurons in these brain regions. These results indicate that CF stress induces intense Fos expression in the noradrenergic LC and serotonergic DR neurons, but not in the dopaminergic VTA neurons. They also indicate that not only monoaminergic neurons but also GABAergic neurons within the LC and DR are activated by the stress. Synapse 45:46–51, 2002. © 2002 Wiley‐Liss, Inc.

Journal

SynapseWiley

Published: Jul 1, 2002

References

  • Tryptophan hydroxylase mRNA levels are elevated by repeated immobilization stress in rat raphe nuclei but not in pineal gland
    Chamas, Chamas; Serova, Serova; Sabban, Sabban
  • Neuroanatomical characterization of Fos induction in rat behavioral models of anxiety
    Duncan, Duncan; Knapp, Knapp; Breese, Breese
  • Potential corticotropin‐releasing factor pathways in the rat brain as determined by bilateral electrolytic lesions of the central amygdaloid nucleus and the paraventricular nucleus of the hypothalamus
    Koegler‐Muly, Koegler‐Muly; Owens, Owens; Ervin, Ervin; Kilts, Kilts; Nemeroff, Nemeroff
  • Extent of colocalization of serotonin and GABA in the neurons of the rat raphe nuclei
    Stamp, Stamp; Semba, Semba

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