Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6 J) tissues

Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine... Monoamines, acting as hormones and neurotransmitters, play a critical role in multiple physiological processes ranging from cognitive function and mood to sympathetic nervous system activity, fight‐or‐flight response and glucose homeostasis. In addition to brain and blood, monoamines are abundant in several tissues, and dysfunction in their synthesis or signaling is associated with various pathological conditions. It was our goal to develop a method to detect these compounds in peripheral murine tissues. In this study, we employed a high‐performance liquid chromatography method using electrochemical detection that allows not only detection of catecholamines but also a detailed analysis of nine monoamines and metabolites in murine tissues. Simple tissue extraction procedures were optimized for muscle (gastrocnemius, extensor digitorum longus and soleus), liver, pancreas and white adipose tissue in the range of weight 10–200 mg. The system allowed a limit of detection between 0.625 and 2.5 pg μL−1 for monoamine analytes and their metabolites, including dopamine, 3,4‐dihydroxyphenylacetic acid, 3‐methoxytyramine, homovanillic acid, norepinephrine, epinephrine, 3‐methoxy‐4‐hydroxyphenylglycol, serotonin and 5‐hydroxyindoleacetic acid. Typical concentrations for different monoamines and their metabolization products in these tissues are presented for C57Bl/6 J mice fed a high‐fat diet. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biomedical Chromatography Wiley

Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6 J) tissues

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons, Ltd.
ISSN
0269-3879
eISSN
1099-0801
D.O.I.
10.1002/bmc.4151
Publisher site
See Article on Publisher Site

Abstract

Monoamines, acting as hormones and neurotransmitters, play a critical role in multiple physiological processes ranging from cognitive function and mood to sympathetic nervous system activity, fight‐or‐flight response and glucose homeostasis. In addition to brain and blood, monoamines are abundant in several tissues, and dysfunction in their synthesis or signaling is associated with various pathological conditions. It was our goal to develop a method to detect these compounds in peripheral murine tissues. In this study, we employed a high‐performance liquid chromatography method using electrochemical detection that allows not only detection of catecholamines but also a detailed analysis of nine monoamines and metabolites in murine tissues. Simple tissue extraction procedures were optimized for muscle (gastrocnemius, extensor digitorum longus and soleus), liver, pancreas and white adipose tissue in the range of weight 10–200 mg. The system allowed a limit of detection between 0.625 and 2.5 pg μL−1 for monoamine analytes and their metabolites, including dopamine, 3,4‐dihydroxyphenylacetic acid, 3‐methoxytyramine, homovanillic acid, norepinephrine, epinephrine, 3‐methoxy‐4‐hydroxyphenylglycol, serotonin and 5‐hydroxyindoleacetic acid. Typical concentrations for different monoamines and their metabolization products in these tissues are presented for C57Bl/6 J mice fed a high‐fat diet.

Journal

Biomedical ChromatographyWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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