Comparison of volumetric capillary cytometry with standard flow cytometry for routine enumeration of CD34+ cells

Comparison of volumetric capillary cytometry with standard flow cytometry for routine enumeration... BACKGROUND: This study assesses the feasibility of a new volumetric cytometry system for the enumeration of CD34+ cells in apheresis components, peripheral blood, and cord blood samples in routine laboratory work. This system is compared with the following flow cytometry protocols: Milan, ISHAGE, ISHAGE with 7‐AAD, and flow‐count fluorospheres. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Comparison of volumetric capillary cytometry with standard flow cytometry for routine enumeration of CD34+ cells

Transfusion, Volume 39 (8) – Jan 1, 1999

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Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services
ISSN
0041-1132
eISSN
1537-2995
DOI
10.1046/j.1537-2995.1999.39080864.x
Publisher site
See Article on Publisher Site

Abstract

BACKGROUND: This study assesses the feasibility of a new volumetric cytometry system for the enumeration of CD34+ cells in apheresis components, peripheral blood, and cord blood samples in routine laboratory work. This system is compared with the following flow cytometry protocols: Milan, ISHAGE, ISHAGE with 7‐AAD, and flow‐count fluorospheres.

Journal

TransfusionWiley

Published: Jan 1, 1999

Keywords: ; ; ; ; ; ;

References

  • Volumetric capillary cytometry: a new method for absolute cell enumeration.
    Dietz, LJ; Dubrow, RS; Manian, BS; Sizto, NL
  • The number of circulating CD34+ blood cells predicts the colony‐forming capacity of leukapheresis products in children.
    Leibundgut, K; Von Rohr, A; Brulhart, K
  • Assessment of interlaboratory variability of immunophenotyping. Results of the College of American Pathologists Flow Cytometry Survey.
    Homburger, H; Rosenstock, W; Paxton, H
  • Variability in absolute lymphocyte counts obtained by automated cell counters.
    Simson, E; Groner, W

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