Comparison of pepsin and trypsin digestion on paraffin‐embedded tissue preparation for DNA flow cytometry

Comparison of pepsin and trypsin digestion on paraffin‐embedded tissue preparation for DNA flow... Preparation with enzymes plays an important part in obtaining good results in cellular DNA content measurements from paraffin‐embedded tissue using flow cytometry. Therefore, we have compared two groups of DNA histograms obtained with pepsin (Hedley method) and trypsin digestion (Schutte method). Samples of five tumor types were compared: eight lung, seven breast, five thyroid, seven stomach, and six colon cancer cases. All samples were stained with propidium iodide (PI). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cytometry Part A Wiley

Comparison of pepsin and trypsin digestion on paraffin‐embedded tissue preparation for DNA flow cytometry

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Publisher
Wiley
Copyright
Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1552-4922
eISSN
1552-4930
DOI
10.1002/cyto.990140514
pmid
8354127
Publisher site
See Article on Publisher Site

Abstract

Preparation with enzymes plays an important part in obtaining good results in cellular DNA content measurements from paraffin‐embedded tissue using flow cytometry. Therefore, we have compared two groups of DNA histograms obtained with pepsin (Hedley method) and trypsin digestion (Schutte method). Samples of five tumor types were compared: eight lung, seven breast, five thyroid, seven stomach, and six colon cancer cases. All samples were stained with propidium iodide (PI).

Journal

Cytometry Part AWiley

Published: Jan 1, 1993

Keywords: ; ; ; ;

References

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