Preparation with enzymes plays an important part in obtaining good results in cellular DNA content measurements from paraffin‐embedded tissue using flow cytometry. Therefore, we have compared two groups of DNA histograms obtained with pepsin (Hedley method) and trypsin digestion (Schutte method). Samples of five tumor types were compared: eight lung, seven breast, five thyroid, seven stomach, and six colon cancer cases. All samples were stained with propidium iodide (PI).
Cytometry Part A – Wiley
Published: Jan 1, 1993
Keywords: ; ; ; ;
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