Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy

Comparative mass measurement of biological macromolecules by scanning transmission electron... A method is described for measuring the mass/length or mass of molecular assemblies by comparative electron scattering in the STEM. Standard particles whose mass is well established (e.g. TMV or fd bacteriophage) are deposited on the electron microscope grid together with the sample to be measured. Images containing at least one sample and standard and with a clean, contamination‐free background are chosen and stored on computer disc and then directly integrated. Use of a comparative technique does not require accurate determination of scattering parameters or instrumental geometry and requires only that the limits of linearity be established. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Microscopy Wiley

Comparative mass measurement of biological macromolecules by scanning transmission electron microscopy

Journal of Microscopy, Volume 122 (3) – Jun 1, 1981

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Publisher
Wiley
Copyright
Copyright © 1981 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-2720
eISSN
1365-2818
DOI
10.1111/j.1365-2818.1981.tb01267.x
Publisher site
See Article on Publisher Site

Abstract

A method is described for measuring the mass/length or mass of molecular assemblies by comparative electron scattering in the STEM. Standard particles whose mass is well established (e.g. TMV or fd bacteriophage) are deposited on the electron microscope grid together with the sample to be measured. Images containing at least one sample and standard and with a clean, contamination‐free background are chosen and stored on computer disc and then directly integrated. Use of a comparative technique does not require accurate determination of scattering parameters or instrumental geometry and requires only that the limits of linearity be established.

Journal

Journal of MicroscopyWiley

Published: Jun 1, 1981

References

  • Regulation of glutamine synthetase, xii. Electron microscopy of the enzyme from E. coli.
    Valentine, R.C.; Shapiro, B.M.; Stadtman, E.R.

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