Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T‐cell lymphomas

Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype... Various enzymatic or mechanical methods have been proposed in the past to dissociate cells from different solid tissues. An automated mechanical disaggregation device (Medimachine™) has recently been proposed. Unfortunately, most of these techniques are associated with a high cellular damage and a low cell recovery and are difficult to apply to skin biopsies. In this paper, we propose a combined enzymatic and mechanical method based on Medimachine™, useful for the isolation of skin infiltrating T‐lymphocytes from small cutaneous biopsies. As this method is easy and allows for a more correct qualitative and quantitative cytofluorimetric analysis of the lymphocyte subsets, it may be useful in the immunophenotyping of cutaneous T‐cell lymphomas. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical & Experimental Dermatology Wiley

Collagenase digestion and mechanical disaggregation as a method to extract and immunophenotype tumour lymphocytes in cutaneous T‐cell lymphomas

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Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0307-6938
eISSN
1365-2230
D.O.I.
10.1046/j.1365-2230.2000.00680.x
Publisher site
See Article on Publisher Site

Abstract

Various enzymatic or mechanical methods have been proposed in the past to dissociate cells from different solid tissues. An automated mechanical disaggregation device (Medimachine™) has recently been proposed. Unfortunately, most of these techniques are associated with a high cellular damage and a low cell recovery and are difficult to apply to skin biopsies. In this paper, we propose a combined enzymatic and mechanical method based on Medimachine™, useful for the isolation of skin infiltrating T‐lymphocytes from small cutaneous biopsies. As this method is easy and allows for a more correct qualitative and quantitative cytofluorimetric analysis of the lymphocyte subsets, it may be useful in the immunophenotyping of cutaneous T‐cell lymphomas.

Journal

Clinical & Experimental DermatologyWiley

Published: Sep 1, 2000

References

  • In search of the central respiratory neurons: Dissociated cell cultures of respiratory areas from the upper medulla
    Fitzgerald, Fitzgerald; Willis, Willis; Yu, Yu; Rigatto, Rigatto
  • Usefulness of the scraping method for DNA flow cytometry in breast tumors
    Cornacchiari, Cornacchiari; Grigolato, Grigolato; Facchetti, Facchetti
  • Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry
    Loken, Loken; Brosnan, Brosnan; Bach, Bach
  • Comparison of pepsin and trypsin digestion on paraffin‐embedded tissue preparation for DNA flow cytometry
    Tagawa, Tagawa; Nakazaky, Nakazaky; Yasutake, Yasutake

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