Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration

Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and... We report the cloning and expression patterns of three novel zebrafish Roundabout homologs. The Roundabout (robo) gene encodes a transmembrane receptor that is essential for axon guidance in Drosophila and Robo family members have been implicated in cell migration. Analysis of extracellular domains and conserved cytoplasmic motifs shows that zebrafish Robo1 and Robo2 are orthologs of mammalian Robo1 and Robo2, respectively, while zebrafish Robo3 is likely to be an ortholog of mouse Rig‐1. The three zebrafish robos are expressed in distinct but overlapping patterns during embryogenesis. They are highly expressed in the developing nervous system, including the olfactory system, visual system, hindbrain, cranial ganglia, spinal cord, and posterior lateral line primordium. They are also expressed in several nonneuronal tissues, including somites and fin buds. The timing and patterns of expression suggest roles for zebrafish robos in axon guidance and cell migration. Wiley‐Liss, Inc. © 2001 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Dynamics Wiley

Cloning and expression of three zebrafish roundabout homologs suggest roles in axon guidance and cell migration

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Publisher
Wiley
Copyright
Copyright © 2001 Wiley‐Liss, Inc.
ISSN
1058-8388
eISSN
1097-0177
DOI
10.1002/dvdy.1136
Publisher site
See Article on Publisher Site

Abstract

We report the cloning and expression patterns of three novel zebrafish Roundabout homologs. The Roundabout (robo) gene encodes a transmembrane receptor that is essential for axon guidance in Drosophila and Robo family members have been implicated in cell migration. Analysis of extracellular domains and conserved cytoplasmic motifs shows that zebrafish Robo1 and Robo2 are orthologs of mammalian Robo1 and Robo2, respectively, while zebrafish Robo3 is likely to be an ortholog of mouse Rig‐1. The three zebrafish robos are expressed in distinct but overlapping patterns during embryogenesis. They are highly expressed in the developing nervous system, including the olfactory system, visual system, hindbrain, cranial ganglia, spinal cord, and posterior lateral line primordium. They are also expressed in several nonneuronal tissues, including somites and fin buds. The timing and patterns of expression suggest roles for zebrafish robos in axon guidance and cell migration. Wiley‐Liss, Inc. © 2001 Wiley‐Liss, Inc.

Journal

Developmental DynamicsWiley

Published: Jun 1, 2001

References

  • The Pfam protein families database
    Bateman, Bateman; Birney, Birney; Durbin, Durbin; Eddy, Eddy; Howe, Howe; Sonnhammer, Sonnhammer
  • Conservation and divergence of axon guidance mechanisms
    Chisholm, Chisholm; Tessier‐Lavigne, Tessier‐Lavigne
  • CDO: an oncogene‐, serum‐, and anchorage‐regulated member of the Ig/fibronectin type III repeat family
    Kang, Kang; Gao, Gao; Feinleib, Feinleib; Cotter, Cotter; Guadagno, Guadagno; Krauss, Krauss
  • Patterning the brain of the zebrafish embryo
    Kimmel, Kimmel
  • Growth cone guidance: first steps towards a deeper understanding
    Mueller, Mueller
  • A novel proline‐rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family
    Niebuhr, Niebuhr; Ebel, Ebel; Frank, Frank; Reinhard, Reinhard; Domann, Domann; Carl, Carl; Walter, Walter; Gertler, Gertler; Wehland, Wehland; Chakraborty, Chakraborty
  • Plasmin‐sensitive dibasic sequences in the third fibronectin‐like domain of L1‐cell adhesion molecule (CAM) facilitate homomultimerization and concomitant integrin recruitment
    Silletti, Silletti; Mei, Mei; Sheppard, Sheppard; Montgomery, Montgomery
  • The DUTT1 gene, a novel NCAM family member is expressed in developing murine neural tissues and has an unusually broad pattern of expression
    Sundaresan, Sundaresan; Roberts, Roberts; Bateman, Bateman; Bankier, Bankier; Sheppard, Sheppard; Hobbs, Hobbs; Xiong, Xiong; Minna, Minna; Latif, Latif; Lerman, Lerman; Rabbitts, Rabbitts
  • Cloning and functional studies of a novel gene aberrantly expressed in RB‐deficient embryos
    Yuan, Yuan; Cox, Cox; Dasika, Dasika; Lee, Lee
  • The mouse SLIT family: secreted ligands for ROBO expressed in patterns that suggest a role in morphogenesis and axon guidance
    Yuan, Yuan; Zhou, Zhou; Chen, Chen; Wu, Wu; Rao, Rao; Ornitz, Ornitz
  • Overexpression of a Slit homologue impairs convergent extension of the mesoderm and causes cyclopia in embryonic zebrafish
    Yeo, Yeo; Little, Little; Yamada, Yamada; Miyashita, Miyashita; Kuwada, Kuwada; Huh, Huh; Okamoto, Okamoto

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