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C. Huang, T. Imamura, G. Moore (1969)
Chromosomes and cloning efficiencies of hematopoietic cell lines derived from patients with leukemia, melanoma, myeloma, and Burkitt lymphoma.Journal of the National Cancer Institute, 43 5
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K. Nilsson, P. Evrin, K. Welsh (1974)
Production of β2‐Microglobulin by Normal and Malignant Human Cell Lines and Peripheral LymphocytesImmunological Reviews, 21
lymphoma cells (Raji). Picture almost identical to the secondarily altered lymphoblasts of Figure 1 B
K. Nilsson, H. Bennich, S. Johansson, J. Pontén (1970)
Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient.Clinical and experimental immunology, 7 4
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Surface Markers on Human B and T LymphocytesScandinavian Journal of Immunology, 3
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Clonal origin for individual Burkitt tumours.Lancet, 1 7653
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HL-A antigens in established human cell lines. Symposium series on immunological standardization
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Long term culture of normal and neoplastic human glia.Acta pathologica et microbiologica Scandinavica, 74 4
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β2-Microglobulin Production in Vitro by Human Hematopoietic, Mesenchymal, and Epithelial CellsThe Journal of Immunology
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Nasopharyngeal carcinoma (NPC). I. Types of cultures derived from tumour biopsies and non‐tumorous tissues of chinese patients with special reference to lymphoblastoid transformationInternational Journal of Cancer, 6
Nilsson Nilsson, Klein Klein, Henle Henle, Henle Henle (1971)
The establishment of lymphoblastoid cell lines and its dependence on EBVInt. J. Cancer, 8
G. Klein, T. Lindahl, M. Jondal, W. Leibold, J. Menezes, K. Nilsson, C. Sundström (1974)
Continuous lymphoid cell lines with characteristics of B cells (bone-marrow-derived), lacking the Epstein-Barr virus genome and derived from three human lymphomas.Proceedings of the National Academy of Sciences of the United States of America, 71 8
K. Nilsson, G. Klein, W. Henle, G. Henle (1971)
The establishment of lymphoblastoid lines from adult and fetal human lymphoid tissue and its dependence on EBVInternational Journal of Cancer, 8
(1973)
Anomalous reactivity of some HL-A typing sera
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Polyclonal origin of lymphoblastoid cell lines established from normal and neoplastic human lymphoid tissues
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Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.Journal of the National Cancer Institute, 49 3
J. Béchet, P. Fialkow, K. Nilsson, G. Klein, S. Singh (1974)
Immunoglobulin synthesis and glucose-6-phosphate dehydrogenase as cell markers in human lymphoblastoid cell lines.Experimental cell research, 89 2
MD BELPOMME, MD MINOWADA, MD MOORE (1972)
Are some human lymphoblastoid cell lines established from leukemic tissues actually derived from normal leukocytes?Cancer, 30
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Chromosomal changes of immunoglobulin-producing cell lines from human lymph nodes with and without lymphoma.Journal of the National Cancer Institute, 41 2
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Characteristics of new cell lines derived from Burkitt lymphomasCancer, 23
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Heterologous transplantation of human hematopoietic cell linesCancer, 25
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cu-lture. A comparative study
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Formal discussion: on the cellular origins and distinctive features of cultured cell lines derived from patients with leukemias and lymphomas.Cancer research, 27 12
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Immunoglobulin synthesis by human reticulum sarcoma cells in vivo and during long-term culture in vitro.Cancer research, 29 4
Burkitt lymphoma line [U-604 BL (Seraphina)]. This line is more strongly attached to feeder cells but few cells are peripoletic
G. Moore, R. Gerner (1970)
Cancer Immunity—Hypothesis and Clinical Trial of Lymphocytotherapy for Malignant DiseasesAnnals of Surgery, 172
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Heterotransplantability of human cell lines derived from leukemia and lymphomas into immunologically tolerant ratsCancer, 24
K. Nilsson, C. Sundström (1974)
Establishment and characteristics of two unique cell lines from patients with lymphosarcomaInternational Journal of Cancer, 13
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Immunoglobulin synthesis in long term cultures of lymph nodes from Hodgkin's disease.Life sciences, 6 24
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Reaction of human smooth muscle antibody with thymus medullary cells.Nature: New biology, 246 152
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Production of β 2 ‐microglobulin by normal and malignant human cell lines and peripheral lymphocytesTransplant Rev., 21
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Lymphoblastoid Transformation and Presence of Herpes-type Viral Particles in a Chinese Nasopharyngeal Tumour cultured in vitroNature, 221
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SURFACE MARKERS ON HUMAN B AND T LYMPHOCYTESThe Journal of Experimental Medicine, 138
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Development of immunocytes and immunoglobulin production in long‐term cultures from normal and malignant human lymph nodesInternational Journal of Cancer, 3
(1971)
Spontaneous lymphoblastoid transformation of human lymphoid tissue or peripheral blood
lymphoma cells (Daudi). Monomorphic morphology. No peripolesis. A few loose clumps
K. Nilsson (1971)
Characteristics of established myeloma and lymphoblastoid cell lines derived from an e myeloma patient: A comparative studyInternational Journal of Cancer, 7
J. Béchet, Kenneth Nilsson, J. Pontén (1973)
Establishment of multiple lymphoblastoid lines from single lymph nodes and study of their immunoglobulin synthesisInternational Journal of Cancer, 11
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Human hematopoietic cells in continuous culture
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Classifi - cation of human lymphoid cells by lectin agglutin - ability and the relationship between neoplasia and degree of agglutination
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P. Fialkow, E. Klein, G. Klein, P. Clifford, Surjit Singh (1973)
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K. Nilsson (1971)
High‐frequency establishment of human immunoglobulin‐producing lymphoblastoid lines from normal and malignant lymphoid tissue and peripheral bloodInternational Journal of Cancer, 8
(1967)
Purification of lymphocytes by flotation
Cell h e derived from patients with myeloma
Minowada Minowada, Ohnuma Ohnuma, Moore Moore (1972)
Brief communication. Rosette‐forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus‐derived lymphocytesJ. nat. Cancer Inst., 49
E. Klein, K. Nilsson, E. Yefenoff (1975)
An established Burkitt's lymphoma line with cell membrane IgG.Clinical immunology and immunopathology, 3 4
Nilsson Nilsson (1971c)
Characteristics of established human myeloma and lymphoblastoid cell lines in long‐term culture. A comparative studyInt. J. Cancer, 7
F. Durr, J. Monroe, R. Schmitter, K. Traul, Y. Hirshaut (1970)
Studies on the infectivity and cytopathology of epstein‐barr virus in human lymphoblastoid cellsInternational Journal of Cancer, 6
R. Furth, H. Gorter, J. Nadkarni, J. Nadkarni, E. Klein, P. Clifford (1972)
Synthesis of immunoglobulins by biopsied tissues and cell lines from Burkitt's lymphoma.Immunology, 22 5
Jondal Jondal, Klein Klein (1973)
Surface markers on human B and T lymphocytes. II. Presence of Epstein‐Barr virus (EBV) receptors on B lymphocytesJ. exp. Med., 138
Klein Klein, Lindahl Lindahl, Jondal Jondal, Leibold Leibold, Menezes Menezes, Nilsson Nilsson, Sundström Sundström (1974)
Continuous lymphoid cell lines with B‐cell characteristics that lack the Epstein‐Barr genome, derived from three human lymphomasProc. nat. Acad. Sci. (Wash.), 71
G. Manolov, Y. Manolova (1972)
Marker Band in One Chromosome 14 from Burkitt LymphomasNature, 237
B. Osunkoya, F. Mottram (1967)
Formal discussion: observations on the establishment of Burkitt's tumor lymphoblasts in serially propagated cultures.Cancer research, 27 12
R. Ham (1963)
An improved nutrient solution for diploid Chinese hamster and human cell lines.Experimental cell research, 29
(1969)
Histopathological definition of Burkitt's tumour.Bulletin of the World Health Organization, 40 4
lymphoma cells (U-47703 BL). Picture as lc and ID except for a higher rate of cell death and differences in cell size. A few adhering cells but no peripolesis
lymphoma line (U-626 BL). Light variations in cell size. A few adhering, but not peripoletic cells
B. Srivastava, Jun Minowada (1973)
Terminal deoxynucleotidyl transferase activity in a cell line (molt-4) derived from the peripheral blood of a patient with acute lymphoblastic leukemia.Biochemical and biophysical research communications, 51 3
G. Miller (1971)
Human lymphoblastoid cell lines and Epstein-Barr virus: a review of their interrelationships and their relevance to the etiology of leukoproliferative states in man.The Yale Journal of Biology and Medicine, 43
MD MOORE, MD KITAMURA, S. Toshima (1968)
Morphology of cultured hematopoietic cellsCancer, 22
Immunological studies on the cell membrane receptors of cultured cells derived from nasopharyngeal cancer , Burkitt ’ s lymphoma and infectious mononucleosis
line 266 BL. Round cells. No clumps. No adherence to feeder cells. Inverted microscope x 100. RPMI 8226 myeloma cells. Variations in cell size. No clumps. No adherence to feeder cells
Monoclonal immunoglobulin production in vitro by established human lymphoid cell lines
Over 200 established human hematopoietic cell lines of normal and malignant origin have been investigated by morphological and functional parameters. Employing morphology as the overriding parameter four types of lines were identified. (1) Lymphoblastoid cell lines, derived from normal and neoplastic hematopoietic tissue, were characterized by the wide morphologic flexibility of individual lymphoblastoid cells, constant association with Epstein‐Barr virus (EBV), polyclonal derivation, differentiation for immunoglobulin production (secretion) and their diploids. (2) Lymphoma cell lines. This type of line was established at a high frequency from Burkitt's lymphoma and rarely from other types of lymphoma, but never from patients without malignancy or with non‐lymphoma malignancies. Important characteristics were morphologic stereotypia within each line, monoclonal derivation, common but not obligatory association with EBV, variability in the expression of Ig synthesis (no production, or membrane bound Ig, or secretion) and aneuploidy. (3) Myeloma cell lines could only rarely be obtained from patients with myeloma. The basis for classification of these lines is their production of Ig identical to the myeloma protein in vitro. Other important distinguishing features were: plasma cell morphology, absence of EBV and aneuploidy. (4) The leukemia cell line (MOLT 4) was the only line with T‐cell characteristics and was easily distinguished from the other types. Important characteristics were a typical surface ultrastructure, absence of EBV and absence of immunoglobulin production. Individual lymphoblastoid lines were in principle identical whereas each line of the other three types had its own characteristic profile. The phenotypic characteristics of the lymphoblastoid lines were very stable during prolonged serial cultivation. Only in a few cases were secondary chromosomal, functional or morphologic alterations noted. We conclude that EBV‐carrying lymphoblastoid lines can be obtained from non‐neoplastic precursor cells from healthy as well as from diseased individuals. Lymphoma, myeloma and leukemia lines are only obtained from the respective neoplastic tissue but generally at a low frequency. With the exception of Burkitt's lymphoma, malignant hematopoietic tissue and leukemia frequently give rise to established cell lines in vitro of the lymphoblastoid type rather than lines derived from the neoplastic cells.
International Journal of Cancer – Wiley
Published: Feb 15, 1975
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