Ciliary Neuronotrophic Factor Stimulates Choline Acetyltransferase Activity in Cultured Chicken Retina Neurons

Ciliary Neuronotrophic Factor Stimulates Choline Acetyltransferase Activity in Cultured Chicken... It has been demonstrated that cultured choliner‐gic retinal neurons from 8‐day‐old chicken embryos respond to a polypeptide factor present in retinal cell‐conditioned medium (RCM) and in retinal extracts. Compared with control cultures, the activity of acetyl‐CoAxholine O‐acetyltransferase (EC 2.3.1.6; ChAT) is enhanced more than twofold in neuronal retinal cultures grown for 7 days in the presence of RCM. The present study demonstrates that both ciliary neuronotrophic factor (CNTF), which is characterized by its trophic activity on parasympathetic ciliary neurons, and RCM exhibit identical stimulatory effects on ChAT activity in retinal monolayer cultures. Similarly, RCM supports the in vitro survival of ciliary neurons to the same extent as CNTF. The active species in RCM has a molecular weight (20,900 ± 1,000) identical to that of CNTF, as determined by preparative sodium dodecyl sul‐fate gel electrophoresis. The results indicate that cholinergic retinal neurons represent a central neuronal target for CNTF or a closely related protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Ciliary Neuronotrophic Factor Stimulates Choline Acetyltransferase Activity in Cultured Chicken Retina Neurons

Journal of Neurochemistry, Volume 51 (1) – Jul 1, 1988

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Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1988.tb04842.x
Publisher site
See Article on Publisher Site

Abstract

It has been demonstrated that cultured choliner‐gic retinal neurons from 8‐day‐old chicken embryos respond to a polypeptide factor present in retinal cell‐conditioned medium (RCM) and in retinal extracts. Compared with control cultures, the activity of acetyl‐CoAxholine O‐acetyltransferase (EC 2.3.1.6; ChAT) is enhanced more than twofold in neuronal retinal cultures grown for 7 days in the presence of RCM. The present study demonstrates that both ciliary neuronotrophic factor (CNTF), which is characterized by its trophic activity on parasympathetic ciliary neurons, and RCM exhibit identical stimulatory effects on ChAT activity in retinal monolayer cultures. Similarly, RCM supports the in vitro survival of ciliary neurons to the same extent as CNTF. The active species in RCM has a molecular weight (20,900 ± 1,000) identical to that of CNTF, as determined by preparative sodium dodecyl sul‐fate gel electrophoresis. The results indicate that cholinergic retinal neurons represent a central neuronal target for CNTF or a closely related protein.

Journal

Journal of NeurochemistryWiley

Published: Jul 1, 1988

References

  • Purification of the chick eye ciliary neuronotrophic factor
    Barbin, Barbin; Manthorpe, Manthorpe
  • New neuronal growth factors
    Berg, Berg
  • A radiochemical method for the determination of choline acetyltransferase
    Fonnum, Fonnum
  • Characterization and partial purification of a novel neuronotrophic factor from bovine seminal vesicle
    Hofmann, Hofmann; Unsicker, Unsicker

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