Chronic Treatment of Rats with SCH‐23390 or Raclopride Does Not Affect the Concentrations of DARPP‐32 or Its mRNA in Dopamine‐Innervated Brain Regions

Chronic Treatment of Rats with SCH‐23390 or Raclopride Does Not Affect the Concentrations of... Abstract: DARPP‐32 (dopamine‐ and cyclic AMP‐regulated phosphoprotein, Mr= 32,000, as determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) is a neuronal phosphoprotein that is enriched in neurons which possess dopamine D1 receptors, particularly striatonigral neurons. In rat brain slices, the phosphorylation state of DARPP‐32 is regulated by dopamine, acting through the dopamine D1 receptor and the adenylyl cyclase system. This study reports that chronic blockade (21 days) of either dopamine D1 receptors by SCH‐23390 or dopamine D2 receptors by raclopride does not affect the concentrations of DARPP‐32 in specific rat brain regions (striatum, thalamus, hippocampus, frontal cerebral cortical pole). Northern blot analysis indicates that the steady‐state level of DARPP‐32 mRNA in striatum is also unchanged by these treatments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Chronic Treatment of Rats with SCH‐23390 or Raclopride Does Not Affect the Concentrations of DARPP‐32 or Its mRNA in Dopamine‐Innervated Brain Regions

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Publisher
Wiley
Copyright
Copyright © 1990 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1990.tb08839.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: DARPP‐32 (dopamine‐ and cyclic AMP‐regulated phosphoprotein, Mr= 32,000, as determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis) is a neuronal phosphoprotein that is enriched in neurons which possess dopamine D1 receptors, particularly striatonigral neurons. In rat brain slices, the phosphorylation state of DARPP‐32 is regulated by dopamine, acting through the dopamine D1 receptor and the adenylyl cyclase system. This study reports that chronic blockade (21 days) of either dopamine D1 receptors by SCH‐23390 or dopamine D2 receptors by raclopride does not affect the concentrations of DARPP‐32 in specific rat brain regions (striatum, thalamus, hippocampus, frontal cerebral cortical pole). Northern blot analysis indicates that the steady‐state level of DARPP‐32 mRNA in striatum is also unchanged by these treatments.

Journal

Journal of NeurochemistryWiley

Published: Jul 1, 1990

References

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