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Chromosome painting as a supplement to cytogenetic banding analysis in non‐Hodgkin's lymphoma

Chromosome painting as a supplement to cytogenetic banding analysis in non‐Hodgkin's lymphoma Chromosomal in situ suppression (CISS) hybridization with biotin labeled chromosome‐specific libraries was performed on short‐term cultures from five cases of non‐Hodgkin's lymphoma (NHL). The painting analysis proceeded in three stages. First‐stage CISS hybridization was done with libraries specific for chromosomes that seemed to be lost or rearranged as judged by banding analysis. Second‐stage CISS included hybridization with probes specific for chromosomes that, because of banding pattern similarities, were considered to be likely candidates to have contributed unidentified chromatin blocks in the abnormal karyotype. The third and final stage was a confirmation hybridization with a library specific for the chromosome that, at the stage two analysis, was found to have donated the previously unknown chromosomal segment. The aberrant chromosomes were often more complex than the banding analysis had led us to believe. Among the rearrangements whose nature was determined by CISS hybridization were two add(1)(p36) which, in both cases, were shown to be a der(1)t(1;2)(p36;q31). This study illustrates the potential use of chromosome painting in resolving karyotypic uncertainties in NHL, and it shows that new cytogenetic subgroups may emerge when classical banding analysis is supplemented with fluorescence in situ hybridization techniques. © 1993 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genes, Chromosomes and Cancer Wiley

Chromosome painting as a supplement to cytogenetic banding analysis in non‐Hodgkin's lymphoma

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References (20)

Publisher
Wiley
Copyright
Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company
ISSN
1045-2257
eISSN
1098-2264
DOI
10.1002/gcc.2870070409
Publisher site
See Article on Publisher Site

Abstract

Chromosomal in situ suppression (CISS) hybridization with biotin labeled chromosome‐specific libraries was performed on short‐term cultures from five cases of non‐Hodgkin's lymphoma (NHL). The painting analysis proceeded in three stages. First‐stage CISS hybridization was done with libraries specific for chromosomes that seemed to be lost or rearranged as judged by banding analysis. Second‐stage CISS included hybridization with probes specific for chromosomes that, because of banding pattern similarities, were considered to be likely candidates to have contributed unidentified chromatin blocks in the abnormal karyotype. The third and final stage was a confirmation hybridization with a library specific for the chromosome that, at the stage two analysis, was found to have donated the previously unknown chromosomal segment. The aberrant chromosomes were often more complex than the banding analysis had led us to believe. Among the rearrangements whose nature was determined by CISS hybridization were two add(1)(p36) which, in both cases, were shown to be a der(1)t(1;2)(p36;q31). This study illustrates the potential use of chromosome painting in resolving karyotypic uncertainties in NHL, and it shows that new cytogenetic subgroups may emerge when classical banding analysis is supplemented with fluorescence in situ hybridization techniques. © 1993 Wiley‐Liss, Inc.

Journal

Genes, Chromosomes and Cancer – Wiley

Published: Aug 1, 1993

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