Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis

Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence... In glucocorticoid‐treated rat thymocytes and the murine lymphoid cell lines L5178 and S49 the morphology of apoptosis is associated with chromatin cleavage. The cleavage is at internucleosomal sites, apparently through activation of an endogenous endonuclease. In variants of the cell lines selected for resistance to glucocorticoid, neither apoptosis nor chromatin cleavage were observed after steroid treatment, and steroid receptors were undetectable. In thymocytes, both the morphological changes of apoptosis and chromatin cleavage were inhibited by cycloheximide and actinomycin D. The calcium–magnesium ionophore A23187 induced apoptosis and chromatin cleavage in thymocytes, and these effects were also inhibited by cycloheximide. The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments. They also provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Pathology Wiley

Chromatin cleavage in apoptosis: Association with condensed chromatin morphology and dependence on macromolecular synthesis

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Publisher
Wiley
Copyright
Copyright © 1984 John Wiley & Sons, Ltd.
ISSN
0022-3417
eISSN
1096-9896
D.O.I.
10.1002/path.1711420112
Publisher site
See Article on Publisher Site

Abstract

In glucocorticoid‐treated rat thymocytes and the murine lymphoid cell lines L5178 and S49 the morphology of apoptosis is associated with chromatin cleavage. The cleavage is at internucleosomal sites, apparently through activation of an endogenous endonuclease. In variants of the cell lines selected for resistance to glucocorticoid, neither apoptosis nor chromatin cleavage were observed after steroid treatment, and steroid receptors were undetectable. In thymocytes, both the morphological changes of apoptosis and chromatin cleavage were inhibited by cycloheximide and actinomycin D. The calcium–magnesium ionophore A23187 induced apoptosis and chromatin cleavage in thymocytes, and these effects were also inhibited by cycloheximide. The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments. They also provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.

Journal

The Journal of PathologyWiley

Published: Jan 1, 1984

References

  • Cell death: the significance of apoptosis
    Wyllie, Wyllie; Kerr, Kerr; Currie, Currie
  • An electron microscopic study of cell degeneration in chick embryo spinal ganglia
    Pannese, Pannese

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