CHO‐Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction

CHO‐Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary... IntroductionChinese hamster ovary cells (CHO) are the expression system of choice in the bioproduction market for the manufacturing of bio‐therapeutic proteins. The first CHO cell lines were established by Puck in 1957, and since then many different lineages were established such as CHO‐S, CHO‐K1, CHOK1SV, DG44, and others. In general, CHO cells are robust, able to grow to high densities in suspension culture while maintaining high viability in large‐scale bioreactors, and can produce protein bio‐therapeutics in the 1–10 g/L range. Typically, stably transfected clones are generated to express bio‐pharmaceutically relevant proteins of interest. Once a cell line is developed, productivity and expression of a specific quality profile can be achieved through process optimization. The goal of such process development work is to maximize yield while maintaining critical quality attributes of the product. This is often achieved through optimization of culture medium, and production processes. Historically, mammalian cell culture medium and feed development has been based on iterative empirical approaches, which are both time consuming and costly. Although development efforts have improved by moving toward high‐throughput component screening in systems that are representative of large‐scale bioproduction processes, there continues to be a financial push to decrease time and effort associated with http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

CHO‐Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction

Loading next page...
 
/lp/wiley/cho-omics-review-the-impact-of-current-and-emerging-technologies-on-by6VUxTGwx
Publisher
Wiley
Copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1860-6768
eISSN
1860-7314
D.O.I.
10.1002/biot.201700227
Publisher site
See Article on Publisher Site

Abstract

IntroductionChinese hamster ovary cells (CHO) are the expression system of choice in the bioproduction market for the manufacturing of bio‐therapeutic proteins. The first CHO cell lines were established by Puck in 1957, and since then many different lineages were established such as CHO‐S, CHO‐K1, CHOK1SV, DG44, and others. In general, CHO cells are robust, able to grow to high densities in suspension culture while maintaining high viability in large‐scale bioreactors, and can produce protein bio‐therapeutics in the 1–10 g/L range. Typically, stably transfected clones are generated to express bio‐pharmaceutically relevant proteins of interest. Once a cell line is developed, productivity and expression of a specific quality profile can be achieved through process optimization. The goal of such process development work is to maximize yield while maintaining critical quality attributes of the product. This is often achieved through optimization of culture medium, and production processes. Historically, mammalian cell culture medium and feed development has been based on iterative empirical approaches, which are both time consuming and costly. Although development efforts have improved by moving toward high‐throughput component screening in systems that are representative of large‐scale bioproduction processes, there continues to be a financial push to decrease time and effort associated with

Journal

Biotechnology JournalWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off