CHO‐Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction

CHO‐Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary... IntroductionChinese hamster ovary cells (CHO) are the expression system of choice in the bioproduction market for the manufacturing of bio‐therapeutic proteins. The first CHO cell lines were established by Puck in 1957, and since then many different lineages were established such as CHO‐S, CHO‐K1, CHOK1SV, DG44, and others. In general, CHO cells are robust, able to grow to high densities in suspension culture while maintaining high viability in large‐scale bioreactors, and can produce protein bio‐therapeutics in the 1–10 g/L range. Typically, stably transfected clones are generated to express bio‐pharmaceutically relevant proteins of interest. Once a cell line is developed, productivity and expression of a specific quality profile can be achieved through process optimization. The goal of such process development work is to maximize yield while maintaining critical quality attributes of the product. This is often achieved through optimization of culture medium, and production processes. Historically, mammalian cell culture medium and feed development has been based on iterative empirical approaches, which are both time consuming and costly. Although development efforts have improved by moving toward high‐throughput component screening in systems that are representative of large‐scale bioproduction processes, there continues to be a financial push to decrease time and effort associated with http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

CHO‐Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1860-6768
eISSN
1860-7314
D.O.I.
10.1002/biot.201700227
Publisher site
See Article on Publisher Site

Abstract

IntroductionChinese hamster ovary cells (CHO) are the expression system of choice in the bioproduction market for the manufacturing of bio‐therapeutic proteins. The first CHO cell lines were established by Puck in 1957, and since then many different lineages were established such as CHO‐S, CHO‐K1, CHOK1SV, DG44, and others. In general, CHO cells are robust, able to grow to high densities in suspension culture while maintaining high viability in large‐scale bioreactors, and can produce protein bio‐therapeutics in the 1–10 g/L range. Typically, stably transfected clones are generated to express bio‐pharmaceutically relevant proteins of interest. Once a cell line is developed, productivity and expression of a specific quality profile can be achieved through process optimization. The goal of such process development work is to maximize yield while maintaining critical quality attributes of the product. This is often achieved through optimization of culture medium, and production processes. Historically, mammalian cell culture medium and feed development has been based on iterative empirical approaches, which are both time consuming and costly. Although development efforts have improved by moving toward high‐throughput component screening in systems that are representative of large‐scale bioproduction processes, there continues to be a financial push to decrease time and effort associated with

Journal

Biotechnology JournalWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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