Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet‐rich plasma and potential use in cell culture

Characterization of wound drainage fluids as a source of soluble factors associated with wound... ABSTRACT Wound fluids, human serum from platelet‐poor and platelet‐rich plasma (SPPP and SPRP), contain various soluble factors involved in cell growth and proliferation. Levels of cytokines, chemokines, and matrix metalloproteinases (MMPs) in drainage fluids (DFs) harvested from subcutaneous wounds, punctured fluids (PF) from seroma, and SPPP were measured. SPPP and SPRP from four healthy volunteers were also subjected to the analysis. Biochemical profiles of DF reflected the sequential stages of wound healing. Early‐phase DF contained high concentrations of basic fibroblast growth factor and platelet‐derived growth factor and EGF. The levels of keratinocyte growth factor, interleukin‐6, and MMP‐8 in DF peaked on days 2–3, while vascular endothelial growth factor, hepatocyte growth factor, interleukin‐8, and MMP‐1 increased over time during days 0–6. Punctured fluids contained high levels of TGF‐β1, keratinocyte growth factor, vascular endothelial growth factor, hepatocyte growth factor, and MMP‐1. Experiments using human adipose‐derived stem cells and dermal fibroblasts cultured in media containing various concentrations of DF and fetal bovine serum suggested that for some cell types, DF‐contained growth factors that are not obtained from SPRP could be used to supplement or substitute for serum in culture media. SPRP and DF are economical ready‐made mixtures of serum and autologous soluble factors, and may be differentially useful for regenerative therapies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Wound Repair and Regeneration Wiley

Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet‐rich plasma and potential use in cell culture

Loading next page...
1
 
/lp/wiley/characterization-of-wound-drainage-fluids-as-a-source-of-soluble-WfvAMJm9um
Publisher
Wiley
Copyright
Copyright © 2007 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1067-1927
eISSN
1524-475X
D.O.I.
10.1111/j.1524-475X.2007.00259.x
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT Wound fluids, human serum from platelet‐poor and platelet‐rich plasma (SPPP and SPRP), contain various soluble factors involved in cell growth and proliferation. Levels of cytokines, chemokines, and matrix metalloproteinases (MMPs) in drainage fluids (DFs) harvested from subcutaneous wounds, punctured fluids (PF) from seroma, and SPPP were measured. SPPP and SPRP from four healthy volunteers were also subjected to the analysis. Biochemical profiles of DF reflected the sequential stages of wound healing. Early‐phase DF contained high concentrations of basic fibroblast growth factor and platelet‐derived growth factor and EGF. The levels of keratinocyte growth factor, interleukin‐6, and MMP‐8 in DF peaked on days 2–3, while vascular endothelial growth factor, hepatocyte growth factor, interleukin‐8, and MMP‐1 increased over time during days 0–6. Punctured fluids contained high levels of TGF‐β1, keratinocyte growth factor, vascular endothelial growth factor, hepatocyte growth factor, and MMP‐1. Experiments using human adipose‐derived stem cells and dermal fibroblasts cultured in media containing various concentrations of DF and fetal bovine serum suggested that for some cell types, DF‐contained growth factors that are not obtained from SPRP could be used to supplement or substitute for serum in culture media. SPRP and DF are economical ready‐made mixtures of serum and autologous soluble factors, and may be differentially useful for regenerative therapies.

Journal

Wound Repair and RegenerationWiley

Published: Jul 1, 2007

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off