Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae)

Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect,... Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26‐0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Insect Biochemistry and Physiology Wiley

Characterization and functional assay of a fatty acyl‐CoA reductase gene in the scale insect, Ericerus pela Chavannes (Hemiptera: Coccoidae)

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0739-4462
eISSN
1520-6327
D.O.I.
10.1002/arch.21445
Publisher site
See Article on Publisher Site

Abstract

Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26‐0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela.

Journal

Archives of Insect Biochemistry and PhysiologyWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

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