Ericerus pela Chavannes (Hemiptera: Coccoidae) is an economically important scale insect because the second instar males secrete a harvestable wax‐like substance. In this study, we report the molecular cloning of a fatty acyl‐CoA reductase gene (EpFAR) of E. pela. We predicted a 520‐aa protein with the FAR family features from the deduced amino acid sequence. The EpFAR mRNA was expressed in five tested tissues, testis, alimentary canal, fat body, Malpighian tubules, and mostly in cuticle. The EpFAR protein was localized by immunofluorescence only in the wax glands and testis. EpFAR expression in High Five insect cells documented the recombinant EpFAR reduced 26‐0:(S) CoA and to its corresponding alcohol. The data illuminate the molecular mechanism for fatty alcohol biosynthesis in a beneficial insect, E. pela.
Archives of Insect Biochemistry and Physiology – Wiley
Published: Jan 1, 2018
Keywords: ; ; ;
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