Characteristics of excitatory amino acid uptake in cultures from neurons and glia from the retina

Characteristics of excitatory amino acid uptake in cultures from neurons and glia from the retina 3H‐D‐Aspartate uptake was biochemically characterized in cultures from chick retina enriched in glial (Müller) cells or neurons during progressive days in vitro (DIV). In the neuronal cultures a high‐affinity, Na+‐dependent system was found with Km = 8–13 μM and pharmacological characteristics in agreement with those of reuptake systems in other regions of the CNS. The uptake system in glial cells showed a lower affinity, with Km = 100–135 μM. In both cases, uptake wastemperature and energy dependent. A sharp increase in the Vmax of uptake was observed in both neuronal and glial cultures at 5 DIV, at which time morphologically mature synapses have been shown to be present in retinal cultures. A parallel increase in the pharmacological specificity of the uptake system in neuronal cultures was observed, with a rise in the efficiency of D‐Asp, L‐Asp, L‐Glu, and DL‐asp‐ β‐hydroxamate for inhibiting 3H‐D‐As‐partate uptake. Results suggest the possibility of re‐uptake participating in the regulation of extracellular glutamate concentration during development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Characteristics of excitatory amino acid uptake in cultures from neurons and glia from the retina

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Publisher
Wiley
Copyright
Copyright © 1991 Wiley‐Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490280412
Publisher site
See Article on Publisher Site

Abstract

3H‐D‐Aspartate uptake was biochemically characterized in cultures from chick retina enriched in glial (Müller) cells or neurons during progressive days in vitro (DIV). In the neuronal cultures a high‐affinity, Na+‐dependent system was found with Km = 8–13 μM and pharmacological characteristics in agreement with those of reuptake systems in other regions of the CNS. The uptake system in glial cells showed a lower affinity, with Km = 100–135 μM. In both cases, uptake wastemperature and energy dependent. A sharp increase in the Vmax of uptake was observed in both neuronal and glial cultures at 5 DIV, at which time morphologically mature synapses have been shown to be present in retinal cultures. A parallel increase in the pharmacological specificity of the uptake system in neuronal cultures was observed, with a rise in the efficiency of D‐Asp, L‐Asp, L‐Glu, and DL‐asp‐ β‐hydroxamate for inhibiting 3H‐D‐As‐partate uptake. Results suggest the possibility of re‐uptake participating in the regulation of extracellular glutamate concentration during development.

Journal

Journal of Neuroscience ResearchWiley

Published: Apr 1, 1991

References

  • The structural specificity of the high affinity uptake of L‐glutamate and L‐aspartate by rat brain slices
    Balcar, Balcar; Johnston, Johnston
  • Effects of chemicals on receptors and horizontal cells in the retina
    Murakami, Murakami; Ohtsu, Ohtsu; Ohtsuka, Ohtsuka
  • High‐affinity uptake of L‐( 3 H)glutamate and D‐( 3 H)aspartate during postnatal development of the hippocampal formation: A quantitative autoradiographic study
    Schmidt, Schmidt; Wolf, Wolf
  • Glutamate‐induced increase in intracellular Ca + + in cerebral cortex neurons is transient in immature cells but permanent in mature cells
    Wahl, Wahl; Schousboe, Schousboe; Honore, Honore; Drejer, Drejer
  • The uptake of L‐glutamate by the retina
    White, White; Neal, Neal

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