Characteristics of established myeloma and lymphoblastoid cell lines derived from an e myeloma patient: A comparative study

Characteristics of established myeloma and lymphoblastoid cell lines derived from an e myeloma... The in vitro characteristics of two types of cell lines established from bone marrow and peripheral blood of an E myeloma patient have been compared. Both types, one with a lymphoblast morphology and the other with plasma cell/plasmablast morphology, secreted monoclonal immunoglobulins in vitro. The former produced IgGK while the latter synthesized the IgEL myeloma protein, which in a previous report was shown to be identical with the myeloma protein in vivo. The myeloma cells were difficult to establish and more stringent in their in vitro requirements than the lymphoblastoid cells. Myeloma cells could only grow in the presence of feeder cells or medium harvested from such cells, while lymphoblasts were capable of independent growth in standard media. The lymphoblastoid line was principally similar to those obtainable from normal lymph nodes (Nilsson et al., 1968; Nilsson, 1971a) and is therefore regarded as being of non‐neoplastic origin. It is thus possible to obtain permanent immunoglobulin‐secreting lines of non‐neoplastic origin as well as myeloma lines of neoplastic origin from patients with myelomatosis. The dynamic and static morphology of the former most closely correspond to the appearance described for lymphoblasts or “immunoblasts”,i.e. lymphocytes stimulated by phyto‐hemagglutinin, while myeloma cells resemble plasma cells of varying maturity. The reasons for the morphologic differences are unknown, but they were sufficiently distinctive to permit unequivocal distinction between the two types of lines. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Cancer Wiley

Characteristics of established myeloma and lymphoblastoid cell lines derived from an e myeloma patient: A comparative study

International Journal of Cancer, Volume 7 (3) – May 15, 1971

Loading next page...
 
/lp/wiley/characteristics-of-established-myeloma-and-lymphoblastoid-cell-lines-ZeRhTvyT4Q
Publisher
Wiley
Copyright
Copyright © 1971 Wiley‐Liss, Inc., A Wiley Company
ISSN
0020-7136
eISSN
1097-0215
DOI
10.1002/ijc.2910070303
Publisher site
See Article on Publisher Site

Abstract

The in vitro characteristics of two types of cell lines established from bone marrow and peripheral blood of an E myeloma patient have been compared. Both types, one with a lymphoblast morphology and the other with plasma cell/plasmablast morphology, secreted monoclonal immunoglobulins in vitro. The former produced IgGK while the latter synthesized the IgEL myeloma protein, which in a previous report was shown to be identical with the myeloma protein in vivo. The myeloma cells were difficult to establish and more stringent in their in vitro requirements than the lymphoblastoid cells. Myeloma cells could only grow in the presence of feeder cells or medium harvested from such cells, while lymphoblasts were capable of independent growth in standard media. The lymphoblastoid line was principally similar to those obtainable from normal lymph nodes (Nilsson et al., 1968; Nilsson, 1971a) and is therefore regarded as being of non‐neoplastic origin. It is thus possible to obtain permanent immunoglobulin‐secreting lines of non‐neoplastic origin as well as myeloma lines of neoplastic origin from patients with myelomatosis. The dynamic and static morphology of the former most closely correspond to the appearance described for lymphoblasts or “immunoblasts”,i.e. lymphocytes stimulated by phyto‐hemagglutinin, while myeloma cells resemble plasma cells of varying maturity. The reasons for the morphologic differences are unknown, but they were sufficiently distinctive to permit unequivocal distinction between the two types of lines.

Journal

International Journal of CancerWiley

Published: May 15, 1971

References

  • Development of immunocytes and immunoglobulin production in long‐term cultures from normal and malignant human lymph nodes
    Nilsson, Nilsson; Pontén, Pontén; Philipson, Philipson
  • Two established in vitro cell lines from human mesenchymal tumours
    Pontén, Pontén; Saksela, Saksela
  • Morphologic, cytogenetic and virologic studies in vitro of a malignant lymphoma from an African child
    Rabson, Rabson; O'Conor, O'Conor; Baron, Baron; Whang, Whang; Legallais, Legallais
  • The lymphocyte
    Trowell, Trowell

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off