Chaotropic Ions Affect the Conformation of Quisqualate Receptors in Rat Cortical Membranes

Chaotropic Ions Affect the Conformation of Quisqualate Receptors in Rat Cortical Membranes Abstract: Binding of (3H)(R,S)‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid ((3H)AMPA) to quisqualate receptors in the presence of SCN ions produced curvilinear Scatchard plots. Kinetic investigations of (3H)‐AMPA binding showed that the curvilinearity cannot be explained by assuming binding to two separate binding sites or by considering it due to cooperative interaction. A more likely explanation is that the quisqualate receptors exist in two states, one with high and one with low affinity for (3H)AMPA. Chaotropic ions change the relaxation constant between the two states. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Chaotropic Ions Affect the Conformation of Quisqualate Receptors in Rat Cortical Membranes

Journal of Neurochemistry, Volume 51 (2) – Aug 1, 1988

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Publisher
Wiley
Copyright
Copyright © 1988 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1988.tb01060.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Binding of (3H)(R,S)‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid ((3H)AMPA) to quisqualate receptors in the presence of SCN ions produced curvilinear Scatchard plots. Kinetic investigations of (3H)‐AMPA binding showed that the curvilinearity cannot be explained by assuming binding to two separate binding sites or by considering it due to cooperative interaction. A more likely explanation is that the quisqualate receptors exist in two states, one with high and one with low affinity for (3H)AMPA. Chaotropic ions change the relaxation constant between the two states.

Journal

Journal of NeurochemistryWiley

Published: Aug 1, 1988

References

  • Selective depression of excitatory amino acid induced depolarizations by magnesium ions in isolated spinal cord preparations
    Ault, Ault; Evans, Evans; Francis, Francis; Oakes, Oakes; Watkins, Watkins
  • The binding of ( 3 H)AMPA, a structural analogue of glutamic acid, to rat brain membranes
    Honore, Honore; Lauridsen, Lauridsen; Krogsgaard‐Larsen, Krogsgaard‐Larsen
  • Excitatory amino acid transmitters
    Watkins, Watkins; Evans, Evans

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