Changes in the Ca 2+ ‐Transport Processes of Red Cells during Cold Storage in ACD

Changes in the Ca 2+ ‐Transport Processes of Red Cells during Cold Storage in ACD In order to characterize Ca2+‐transport in red cells stored in ACD Ca2+‐loading and Mg2+‐depletion by the ionophore A23187, CaATPase activity determination in intact cells and an accurate Ca2+‐influx technique were adapted to preserved blood. Active Ca2+‐efflux (pump) was measured in rejuvenated cells loaded by Ca2+ with A23187. The rate of Ca2+‐pump declined only slightly during 3 weeks of storage (from 80 ± 15 to 66 ± 17 μmoles Ca2+/l. cells/min) and a marginal trend of decrease in the Ca:ATP ratio was observed (from 1.96 ± 0.15 to 1.88±0.11). Passive Ca2+‐influx (leak) was studied in regenerated cells in which the Ca2+‐pump was blocked with 0.2 mm lanthanum. Ca2+‐influx showed a slow increase during the first 3 weeks of storage (from 0.4±0.16 to 1.25±0.4 μmoles Ca2+/l. cells/min), later it increased rapidly. Passive Ca2+ leak and exchange transport were studied in unregenerated, phosphate ester‐depleted cells. In this case passive Ca2+‐influx increased 2–3‐fold as early as 1–2 d after storage in ACD. This initial increase was followed by a continuous slow enhancement that reached a flux of 3.5±0.7 μmoles Ca2+/l. cells/min after 3 weeks of storage. The passive Ca2+‐permeability increase that occurred during storage could be readily compensated by the Ca2+‐pump without causing metabolic imbalance. The Ca2+‐transport, of unregenerated stored cells, however, showed impairment under certain conditions (A23187+EDTA and lanthanum treatments, ghost preparation). The Ca2+‐induced shape changes were reversible and ran parallel with the cell Ca2+ level during Ca2+‐pumping up to 5 weeks of storage. This finding indicates a direct relationship between cell Ca2+ and shape. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Haematology Wiley

Changes in the Ca 2+ ‐Transport Processes of Red Cells during Cold Storage in ACD

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Publisher
Wiley
Copyright
Copyright © 1978 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0007-1048
eISSN
1365-2141
D.O.I.
10.1111/j.1365-2141.1978.tb03626.x
Publisher site
See Article on Publisher Site

Abstract

In order to characterize Ca2+‐transport in red cells stored in ACD Ca2+‐loading and Mg2+‐depletion by the ionophore A23187, CaATPase activity determination in intact cells and an accurate Ca2+‐influx technique were adapted to preserved blood. Active Ca2+‐efflux (pump) was measured in rejuvenated cells loaded by Ca2+ with A23187. The rate of Ca2+‐pump declined only slightly during 3 weeks of storage (from 80 ± 15 to 66 ± 17 μmoles Ca2+/l. cells/min) and a marginal trend of decrease in the Ca:ATP ratio was observed (from 1.96 ± 0.15 to 1.88±0.11). Passive Ca2+‐influx (leak) was studied in regenerated cells in which the Ca2+‐pump was blocked with 0.2 mm lanthanum. Ca2+‐influx showed a slow increase during the first 3 weeks of storage (from 0.4±0.16 to 1.25±0.4 μmoles Ca2+/l. cells/min), later it increased rapidly. Passive Ca2+ leak and exchange transport were studied in unregenerated, phosphate ester‐depleted cells. In this case passive Ca2+‐influx increased 2–3‐fold as early as 1–2 d after storage in ACD. This initial increase was followed by a continuous slow enhancement that reached a flux of 3.5±0.7 μmoles Ca2+/l. cells/min after 3 weeks of storage. The passive Ca2+‐permeability increase that occurred during storage could be readily compensated by the Ca2+‐pump without causing metabolic imbalance. The Ca2+‐transport, of unregenerated stored cells, however, showed impairment under certain conditions (A23187+EDTA and lanthanum treatments, ghost preparation). The Ca2+‐induced shape changes were reversible and ran parallel with the cell Ca2+ level during Ca2+‐pumping up to 5 weeks of storage. This finding indicates a direct relationship between cell Ca2+ and shape.

Journal

British Journal of HaematologyWiley

Published: Aug 1, 1978

References

  • The control by internal calcium of membrane permeability to sodium and potassium
    Romero, Romero; Whittam, Whittam
  • Calcium movements across the membrane of human red cells
    Schatzmann, Schatzmann; Vincenzi, Vincenzi
  • On the mechanism of ATP‐induced shape changes in human erythrocyte membranes. I. The role of the spectrin complex
    Sheetz, Sheetz; Singer, Singer
  • Active calcium ion uptake by inside‐out and right side‐out vesicles of red blood cell membranes
    Weiner, Weiner; Lee, Lee

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