Changes in the Activities of some Membrane‐associated Enzymes during in Vivo Ageing of the Normal Human Erythrocyte

Changes in the Activities of some Membrane‐associated Enzymes during in Vivo Ageing of the... Summary. Human erythrocytes from healthy male donors were fractionated with respect to in vivo age by simple centrifugation in order to characterize changes in the functional integrity of the membrane during the life‐span of the cell. The three enzymes, Na/K‐ATPase, glyceraldehyde‐3‐phosphate dehydrogenase and NADH‐ferricyanide reductase, were found not to change with age, but significant age‐dependent decreases were observed in the cases of acetylcholinesterase, phosphoglycerate kinase, purine nucleoside phosphorylase, adenylate kinase, Mg‐ATPase and alkaline phosphatase. The possibility mat these changes were attributable to mechanisms other than age‐related inactivation, such as reticulocyte contamination, differential resealing and crypticity, was investigated. Only the decrease in acetylcholinesterase could be explained wholly in terms of reticulocyte contamination. A decrease in membrane integrity on ageing was observed, which accounted for approximately half the change in alkaline phosphatase and may have contributed to the other enzyme activity changes. This membrane integrity effect masked a real decrease in the highly cryptic NADH‐ferricyanide reductase, this decrease being apparent only after total disaggregation of the membrane with nonionic surfactant. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Haematology Wiley

Changes in the Activities of some Membrane‐associated Enzymes during in Vivo Ageing of the Normal Human Erythrocyte

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Abstract

Summary. Human erythrocytes from healthy male donors were fractionated with respect to in vivo age by simple centrifugation in order to characterize changes in the functional integrity of the membrane during the life‐span of the cell. The three enzymes, Na/K‐ATPase, glyceraldehyde‐3‐phosphate dehydrogenase and NADH‐ferricyanide reductase, were found not to change with age, but significant age‐dependent decreases were observed in the cases of acetylcholinesterase, phosphoglycerate kinase, purine nucleoside phosphorylase, adenylate kinase, Mg‐ATPase and alkaline phosphatase. The possibility mat these changes were attributable to mechanisms other than age‐related inactivation, such as reticulocyte contamination, differential resealing and crypticity, was investigated. Only the decrease in acetylcholinesterase could be explained wholly in terms of reticulocyte contamination. A decrease in membrane integrity on ageing was observed, which accounted for approximately half the change in alkaline phosphatase and may have contributed to the other enzyme activity changes. This membrane integrity effect masked a real decrease in the highly cryptic NADH‐ferricyanide reductase, this decrease being apparent only after total disaggregation of the membrane with nonionic surfactant.

Journal

British Journal of HaematologyWiley

Published: Sep 1, 1977

References

  • The determination of the length of life of the transfused blood corpuscles in man
    Ashby, Ashby
  • Red cell membrane protein in antibody induced haemolytic anaemia
    Gomperts, Gomperts; Metz, Metz; Zail, Zail
  • Studies of the role of red cell membrane peroxidation in paroxysmal nocturnal haemoglobinuria (PNH)
    Paniker, Paniker; Arnold, Arnold; Hartmann, Hartmann
  • The organization of proteins in the human red blood cell membrane. A review
    Steck, Steck
  • Some lipid characteristics of normal red blood cells of different age
    Gastel, Gastel; Berg, Berg; Gier, Gier; Deenen, Deenen

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